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प्रश्न
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
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उत्तर
- Palindromic sequences, endonuclease enzyme
- Restriction enzymes can make complementary cut counterparts forming sticky ends for recombination DNA/RDNA technology to facilitate the ligation of vector and foreign DNA.
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संबंधित प्रश्न
Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
DNA fragments separate according to size through?
Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?
'Restriction' in Restriction enzyme refers to ______.
What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
State the importance of elution in this process.
In the restriction endonuclease EcoRI, what do the letter 'R' and the Roman numeral 'I' represent?
How are separated DNA bands visualized following agarose gel electrophoresis?
