Advertisements
Advertisements
प्रश्न
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
Advertisements
उत्तर
- Palindromic sequences, endonuclease enzyme
- Restriction enzymes can make complementary cut counterparts forming sticky ends for recombination DNA/RDNA technology to facilitate the ligation of vector and foreign DNA.
APPEARS IN
संबंधित प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
Suggest a technique to a researcher who needs to separate fragments of DNA.
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
DNA fragments separate according to size through?
While isolating DNA from bacteria, which of the following enzymes is not required?
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
Identify the activity of endonuclease and exonuclease in the given image.
