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प्रश्न
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- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
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उत्तर
- Palindromic sequences, endonuclease enzyme
- Restriction enzymes can make complementary cut counterparts forming sticky ends for recombination DNA/RDNA technology to facilitate the ligation of vector and foreign DNA.
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संबंधित प्रश्न
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
Distinguish between exonuclease and endonuclease.
The total number of nucleotide sequences of DNA that code for a hormone is 1530. The proportion of different bases in the sequence is found to be Adenine = 34%, Guanine = 19%, Cytosine = 23%, Thymine = 19%.
Applying Chargaff’s rule, what conclusion can be drawn?
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
DNA fragments separate according to size through?
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
What is elution?
