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प्रश्न
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
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उत्तर
- Palindromic sequences, endonuclease enzyme
- Restriction enzymes can make complementary cut counterparts forming sticky ends for recombination DNA/RDNA technology to facilitate the ligation of vector and foreign DNA.
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संबंधित प्रश्न
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
How does restriction endonuclease function?
The total number of nucleotide sequences of DNA that code for a hormone is 1530. The proportion of different bases in the sequence is found to be Adenine = 34%, Guanine = 19%, Cytosine = 23%, Thymine = 19%.
Applying Chargaff’s rule, what conclusion can be drawn?
Which of the following radioisotope is not suitable for DNA labeling based studies?
Molecular scissors, which cut DNA at specific site is ______.
'Restriction' in Restriction enzyme refers to ______.
Would you choose an exonuclease while producing a recombinant DNA molecule?
What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?
What is elution?
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
