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प्रश्न
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- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
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उत्तर
- Palindromic sequences, endonuclease enzyme
- Restriction enzymes can make complementary cut counterparts forming sticky ends for recombination DNA/RDNA technology to facilitate the ligation of vector and foreign DNA.
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संबंधित प्रश्न
Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
The total number of nucleotide sequences of DNA that code for a hormone is 1530. The proportion of different bases in the sequence is found to be Adenine = 34%, Guanine = 19%, Cytosine = 23%, Thymine = 19%.
Applying Chargaff’s rule, what conclusion can be drawn?
Which of the following radioisotope is not suitable for DNA labeling based studies?
Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?
'Restriction' in Restriction enzyme refers to ______.
Which of the following bacteria is not a source of restriction endonuclease?
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
How does one visualise DNA on an agarose gel?
According to the sieving effect of an agarose gel matrix, which DNA fragments move the farthest and fastest from the loading wells?
