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प्रश्न
Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?
विकल्प
Endonuclease
Exonuclease
DNA ligase
Hind - II
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उत्तर
Exonuclease
Explanation:
The enzyme that catalyzes the removal of nucleotides from the ends of DNA is exonuclease. Exonuclease specifically removes nucleotides one by one from the ends of a DNA strand, whereas endonucleases cut DNA at internal sites. DNA ligase joins DNA fragments but does not remove nucleotides. Hind II is a type of restriction endonuclease that cuts DNA at specific internal sequences, not at the ends.
संबंधित प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
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How does a restriction nuclease function? Explain
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Answer the following question.
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Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
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(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.
What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
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- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
In the restriction endonuclease EcoRI, what do the letter 'R' and the Roman numeral 'I' represent?
Which of the following represents the specific palindromic recognition sequence cleaved by the restriction endonuclease EcoRI?
What is the origin of agarose, the matrix most commonly used in gel electrophoresis?
According to the sieving effect of an agarose gel matrix, which DNA fragments move the farthest and fastest from the loading wells?
Why are Type II restriction endonucleases the primary class of restriction enzymes used in genetic manipulation?
