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प्रश्न
Which of the following bacteria is not a source of restriction endonuclease?
विकल्प
Haemophilus influenzae
Escherichia coli
Entamoeba coli
Bacillus amyloliquefaciens
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उत्तर
Entamoeba coli
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संबंधित प्रश्न
How does restriction endonuclease function?
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
A mixture containing DNA fragments a, b, c and d, with molecular weights of a + b = c, a > b and d > c was subject to agarose get electrophoresis. This position of these fragments from cathode to anode to anode sides of the gel would be ______.
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
'Restriction' in restriction enzyme refers to
DNA fragments separate according to size through?
Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
Would you choose an exonuclease while producing a recombinant DNA molecule?
What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
What is the precise definition of a palindromic sequence in DNA?
According to the sieving effect of an agarose gel matrix, which DNA fragments move the farthest and fastest from the loading wells?
How are separated DNA bands visualized following agarose gel electrophoresis?
What is the process called when desired, separated DNA bands are physically cut out from the agarose gel and extracted from the gel piece?
