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प्रश्न
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
विकल्प
Charge only
Size only
Charge to size ratio
All of the above
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उत्तर
In agarose gel electrophoresis, DNA molecules are separated on the basis of their size only.
Explanation:
The gel acts as a molecular sieve, where smaller DNA fragments migrate faster through the pores of the agarose matrix compared to larger ones. Although DNA molecules are negatively charged and move toward the positive electrode due to their charge, the separation principle hinges predominantly on size differences, not charge alone or charge-to-size ratio.
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संबंधित प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
How does a restriction nuclease function? Explain
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Do eukaryotic cells have restriction endonucleases? Justify your answer.
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
How does restriction endonuclease function?
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
Give a reason why :
Single cloning site is preferred in a vector.
Restriction enzymes ______.
Molecular scissors, which cut DNA at specific site is ______.
'Restriction' in restriction enzyme refers to
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
Which of the following statements does not hold true for restriction enzyme?
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
How are DNA fragments visualised once they are separated by gel electrophoresis?
