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प्रश्न
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
विकल्प
Charge only
Size only
Charge to size ratio
All of the above
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उत्तर
In agarose gel electrophoresis, DNA molecules are separated on the basis of their size only.
Explanation:
The gel acts as a molecular sieve, where smaller DNA fragments migrate faster through the pores of the agarose matrix compared to larger ones. Although DNA molecules are negatively charged and move toward the positive electrode due to their charge, the separation principle hinges predominantly on size differences, not charge alone or charge-to-size ratio.
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संबंधित प्रश्न
Mention the difference in the mode of action of exonuclease and endonuclease.
Explain briefly:
Restriction enzymes and DNA
How does restriction endonuclease function?
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
Molecular scissors, which cut DNA at specific site is ______.
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?
While isolating DNA from bacteria, which of the following enzymes is not required?
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
Would you choose an exonuclease while producing a recombinant DNA molecule?
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.
(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.
What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
State the importance of elution in this process.
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
State the principle involved in separation of DNA fragments using gel electrophoresis.
