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प्रश्न
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
विकल्प
Charge only
Size only
Charge to size ratio
All of the above
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उत्तर
In agarose gel electrophoresis, DNA molecules are separated on the basis of their size only.
Explanation:
The gel acts as a molecular sieve, where smaller DNA fragments migrate faster through the pores of the agarose matrix compared to larger ones. Although DNA molecules are negatively charged and move toward the positive electrode due to their charge, the separation principle hinges predominantly on size differences, not charge alone or charge-to-size ratio.
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संबंधित प्रश्न
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Suggest a technique to a researcher who needs to separate fragments of DNA.
Explain briefly:
Restriction enzymes and DNA
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Restriction Enzymes
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
A mixture containing DNA fragments a, b, c and d, with molecular weights of a + b = c, a > b and d > c was subject to agarose get electrophoresis. This position of these fragments from cathode to anode to anode sides of the gel would be ______.
Which of the following radioisotope is not suitable for DNA labeling based studies?
DNA fragments separate according to size through?
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?
While isolating DNA from bacteria, which of the following enzymes is not required?
Would you choose an exonuclease while producing a recombinant DNA molecule?
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
What is the origin of agarose, the matrix most commonly used in gel electrophoresis?
How are separated DNA bands visualized following agarose gel electrophoresis?
