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प्रश्न
The most important feature in a plasmid to serve as a vector in gene cloning experiment is ______.
विकल्प
Origin of replication (ori)
Presence of a selectable marker
Presence of sites for restriction endonuclease
Its size
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उत्तर
The most important feature in a plasmid to serve as a vector in gene cloning experiment is origin of replication (ori).
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संबंधित प्रश्न
Retroviruses have no DNA. However, the DNA of the infected host cell does possess viral DNA. How is it possible?
Rearrange the following in the correct sequence to accomplish an important biological reaction:
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA
State the role of DNA ligase in biotechnology.
Give an account of the Blue-White Method of selection of recombinants.
Give one significant difference between:
Electroporation and Gene Gun.
What is Palindromic sequence?
What is the role of Taq-polymerase in PCR technology?
For which of the following purpose restriction endonuclease enzyme is used in genetic engineering?
The time taken to complete one cycle of PCR is around ______.
Identify the properties that a cloning vector must possess to be efficiently used in rDNA technology.
- Control elements like promoter, operator, ribosomal binding sites
- Restriction sites
- Marker genes
- Ori of replication
- Ability to replicate independently
____________ cuts the DNA within the specific positions.
What is true for plasmid?
Which of the following is not required in the preparation of a recombinant DNA molecule?
What is meant by gene cloning?
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
What is polymerase chain reaction (PCR)?
What is EcoRI?
What is the function of DNA ligase in the creation of recombinant DNA?
The three basic steps in genetic modification are:
The origin of replication is best described as:
