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प्रश्न
The most important feature in a plasmid to serve as a vector in gene cloning experiment is ______.
विकल्प
Origin of replication (ori)
Presence of a selectable marker
Presence of sites for restriction endonuclease
Its size
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उत्तर
The most important feature in a plasmid to serve as a vector in gene cloning experiment is origin of replication (ori).
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संबंधित प्रश्न
What would be the molar concentration of human DNA in a human cell?
The technique which involves addition or deletion of genes is ______.
What is Palindromic sequence?
Which of the following device is required for PCR?
Identify the properties that a cloning vector must possess to be efficiently used in rDNA technology.
- Control elements like promoter, operator, ribosomal binding sites
- Restriction sites
- Marker genes
- Ori of replication
- Ability to replicate independently
Match the column I (PCR steps) and Column II (Temperature). Select the correct option.
| Column I (PCR steps) | Column II (Temperature) | ||
| i. | Polymerization | P. | 40 - 60°C |
| ii. | Denaturation | Q. | 70 - 75°C |
| iii. | Primer annealing | R. | 90 - 98°C |
| S. | 28 - 37°C |
Exonuclease performs the function of ____________.
Human insulin is produced by culturing the genetically engineered ______
The main reason for the presence of both a leading and a lagging strand during DNA replication is, ______
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
Restriction enzymes were discovered by ______.
Which of these are most widely used in genetic engineering?
Which of the following statements are true about cloning vectors?
- Vectors are DNA molecules that carry a foreign DNA segment.
- The replicated DNA is introduced into the host cell.
- It possesses the origin of replication (Ori).
- Vectors may be plasmid, bacteriophage, Yeast artificial chromosomes (YACs), etc.
- Vectors do not require a selectable marker to identify and eliminate non-transformants.
Choose the correct answer from the options given below.
Study the following lists and choose the correct match.
| List I | List II |
| A. Vector | (i) Resistant to cotton bollworms |
| B. Downstream processing | (ii) Mobile genetic elements |
| C. Cry II AB | (iii) Controls corn borers |
| D. Transposons | (iv) Ti plasmid |
| (v) Purifying protein in biopharmaceuticals |
To be a cloning vector, a plasmid require ______.
Which amino acid is coded by the initiation codon?
Expand YAC used in the field of Biotechnology.
Name the source of thermostable DNA polymerase.
Give a reason for the following:
DNA cannot enter directly into the host cell.
