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प्रश्न
The most important feature in a plasmid to serve as a vector in gene cloning experiment is ______.
विकल्प
Origin of replication (ori)
Presence of a selectable marker
Presence of sites for restriction endonuclease
Its size
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उत्तर
The most important feature in a plasmid to serve as a vector in gene cloning experiment is origin of replication (ori).
APPEARS IN
संबंधित प्रश्न
Give the applications of the PCR technique.
In vitro amplification of DNA or RNA segment is known as ___________.
The technique which involves addition or deletion of genes is ______.
What is plasmid?
Identify the palindromic sequence which is recognized by the EcoRI restriction enzyme.
From the following which one is NOT a feature of the plasmids?
Identify the properties that a cloning vector must possess to be efficiently used in rDNA technology.
- Control elements like promoter, operator, ribosomal binding sites
- Restriction sites
- Marker genes
- Ori of replication
- Ability to replicate independently
____________ cuts the DNA within the specific positions.
Exonuclease performs the function of ____________.
Human insulin is produced by culturing the genetically engineered ______
Which of the following is the correct recognition sequence of restriction enzyme Eco RI?
Which of the following statements are true about cloning vectors?
- Vectors are DNA molecules that carry a foreign DNA segment.
- The replicated DNA is introduced into the host cell.
- It possesses the origin of replication (Ori).
- Vectors may be plasmid, bacteriophage, Yeast artificial chromosomes (YACs), etc.
- Vectors do not require a selectable marker to identify and eliminate non-transformants.
Choose the correct answer from the options given below.
Describe three steps involved in mechanism of PCR.
pBR322, used very frequently in cloning experiments as a vector in E. coli is ______.
______technique is used for in vitro gene cloning or gene multiplication.
What are the different types of electrophoresis?
Tissue plasminogen activator and urokinase are used in the treatment of ______.
Name the enzyme responsible for the transcription of genetic code to produce mRNA.
Name the enzymes that are called molecular scissors.
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
-
Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
