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प्रश्न
The most important feature in a plasmid to serve as a vector in gene cloning experiment is ______.
विकल्प
Origin of replication (ori)
Presence of a selectable marker
Presence of sites for restriction endonuclease
Its size
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उत्तर
The most important feature in a plasmid to serve as a vector in gene cloning experiment is origin of replication (ori).
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संबंधित प्रश्न
What are plasmids?
Name and explain the technique used for separating DNA fragments and making them available for biotechnology experiments.
Why are bio-fertilizers preferred over chemical fertilizers?
Enlist and write in brief about the different biological tools required in rDNA technology.
What is gene cloning? Explain different tools used for it.
From the following list of cloning vectors used in rDNA technology identify the vectors that are the most commonly used.
Plasmids, bacteriophages (Ml3, lambda virus), cosmid, phagemid, BAC (bacterial artificial chromosome), YAC (yeast artificial chromosome), transposons, baculoviruses, and mammalian artificial chromosomes (MACs)
Consider there is only one double stranded DNA molecule initially along with all other requirements of PCR. If a PCR machines performs 10 cycles of reaction then bow many double stranded DNA molecules would be present at the end?
From which of the following organisms the enzyme Taq polymerase used in PCR technique is obtained?
______ is a Type I restriction enzyme.
Following are the four statements regarding separation of DNA fragments using Gel electrophoresis. Identify the INCORRECT statements.
i. DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal.
ii. DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
iii. Smaller the size of DNA fragment, larger is the distance it travels through it.
iv. Pure DNA can be visualized directly by exposing to UV radiation.
____________ is the manipulation of genetic material towards a desired end, in a directed and predetermined way, using in vitro process.
In PCR technique, the organism providing thermostable DNA polymerase is a ____________.
What is the significance of adding proteases at the time of isolation of genetic material (DNA)?
Annealing step of PCR operates at ______ °C.
Expand the following acronym:
BAC
Study the following lists and choose the correct match.
| List I | List II |
| A. Vector | (i) Resistant to cotton bollworms |
| B. Downstream processing | (ii) Mobile genetic elements |
| C. Cry II AB | (iii) Controls corn borers |
| D. Transposons | (iv) Ti plasmid |
| (v) Purifying protein in biopharmaceuticals |
To be a cloning vector, a plasmid require ______.
How gene amplification is done?
Write steps involved in gene amplification.
Name the source of thermostable DNA polymerase.
