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प्रश्न
The most important feature in a plasmid to serve as a vector in gene cloning experiment is ______.
पर्याय
Origin of replication (ori)
Presence of a selectable marker
Presence of sites for restriction endonuclease
Its size
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उत्तर
The most important feature in a plasmid to serve as a vector in gene cloning experiment is origin of replication (ori).
APPEARS IN
संबंधित प्रश्न
Give the applications of the PCR technique.
Give one significant difference between:
Electroporation and Gene Gun.
Enlist and write in brief about the different biological tools required in rDNA technology.
The following identifies the major features of technology that differentiate modem biotechnology from classical biotechnology.
i. Capability of science to change the genetic material for getting new specific products through rDNA technology, polymerase chain reaction (PCR), microarrays, cell culture and fusion, and bioprocessing.
ii. Ownership of technology and its sociopolitical impact.
iii. Modem biotechnology makes use of only fermentation technology.
iv. Classical biotechnology relies only on principles of plant and animal tissue culture.
From which of the following organisms the enzyme Taq polymerase used in PCR technique is obtained?
Exonuclease performs the function of ____________.
The rDNA technology has provided a method to control the nematode parasite Meloidogyne incognita. Explain the principle involved in this technique.
Nucleic acid segment tagged with a radioactive molecule is called ______.
Which of the following is the correct recognition sequence of restriction enzyme Eco RI?
Which vector is mostly used in rDNA technology in plants?
Identify the desirable characteristics for a plasmid used in rDNA technology from the following.
- Ability to multiply and express outside the host in a bioreactor.
- A highly active promoter.
- A site at which replication can be initiated.
- One or more identifiable marker genes.
- One or more unique restriction sites.
What is polymerase chain reaction (PCR)?
Describe three steps involved in mechanism of PCR.
To be a cloning vector, a plasmid require ______.
What are the different types of electrophoresis?
Explain the mechanism of PCR.
How many sets of primers are required in each cycle of PCR?
Give a reason for the following:
DNA cannot enter directly into the host cell.
The restriction enzymes used as molecular scissors are type of ______.
