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प्रश्न
The most important feature in a plasmid to serve as a vector in gene cloning experiment is ______.
पर्याय
Origin of replication (ori)
Presence of a selectable marker
Presence of sites for restriction endonuclease
Its size
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उत्तर
The most important feature in a plasmid to serve as a vector in gene cloning experiment is origin of replication (ori).
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संबंधित प्रश्न
Retroviruses have no DNA. However, the DNA of the infected host cell does possess viral DNA. How is it possible?
How has the development of bioreactor helped in biotechnology?
Give the applications of the PCR technique.
What is Palindromic sequence?
Define biotechnology? Which are the basic principles and process of biotechnology?
What is PCR? Explain different steps involved in it.
Match the List I with List II and List ill. Select the correct option.
| List I | List II | List ill | |||
| i. | Hind III | a. | Agarose ge | 1. | Six base pairs |
| ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marke |
| iii. | T-DNA | c. | Ampicillin | 3. | Elution |
| iv. | DNA | d. | Recognition sequenc | 4. | Transgenic plant |
The following identifies the major features of technology that differentiate modem biotechnology from classical biotechnology.
i. Capability of science to change the genetic material for getting new specific products through rDNA technology, polymerase chain reaction (PCR), microarrays, cell culture and fusion, and bioprocessing.
ii. Ownership of technology and its sociopolitical impact.
iii. Modem biotechnology makes use of only fermentation technology.
iv. Classical biotechnology relies only on principles of plant and animal tissue culture.
Consider there is only one double stranded DNA molecule initially along with all other requirements of PCR. If a PCR machines performs 10 cycles of reaction then bow many double stranded DNA molecules would be present at the end?
Study the following statements regarding separation of DNA using agarose gel electrophoresis.
- Large fragments of DNA molecules move faster than small DNA fragments and thus separate faster.
- DNA being negatively charged migrates to cathode.
The main reason for the presence of both a leading and a lagging strand during DNA replication is, ______
Which molecules are called molecular scissors? Why are they called so?
Which of the following statements is correct with respect to cellular respiration?
The source of taq Polymerase used in PCR is a ______.
DNA fingerprinting refers to ______.
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
Which of these are most widely used in genetic engineering?
Study the following lists and choose the correct match.
| List I | List II |
| A. Vector | (i) Resistant to cotton bollworms |
| B. Downstream processing | (ii) Mobile genetic elements |
| C. Cry II AB | (iii) Controls corn borers |
| D. Transposons | (iv) Ti plasmid |
| (v) Purifying protein in biopharmaceuticals |
State the steps involved in the process of gene therapy for the treatment of ADA-deficiency.
