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प्रश्न
Which molecules are called molecular scissors? Why are they called so?
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उत्तर
Restriction enzymes are called molecular scissors.
Each restriction endonuclease inspects the length of a DNA sequence. Once it finds its specific recognition sequence, it will bind to the DNA and cut each of the two strands of the double helix at specific points in their sugar-phosphate backbones. Each restriction endonuclease recognises specific palindromic nucleotide sequences in the DNA.
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संबंधित प्रश्न
Give one significant difference between:
Electroporation and Gene Gun.
The enzyme restriction endonuclease ______.
Identify the characterization techniques that are based on molecular weights of compounds.
The gene transfer between unrelated plants is brought about by ____________.
Lytic cycle involves bacteriophage and ____________.
Human insulin is produced by culturing the genetically engineered ______
Name a recombinant vaccine that is currently being used in vaccination programme.
Annealing step of PCR operates at ______ °C.
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
To be a cloning vector, a plasmid require ______.
