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प्रश्न
Which molecules are called molecular scissors? Why are they called so?
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उत्तर
Restriction enzymes are called molecular scissors.
Each restriction endonuclease inspects the length of a DNA sequence. Once it finds its specific recognition sequence, it will bind to the DNA and cut each of the two strands of the double helix at specific points in their sugar-phosphate backbones. Each restriction endonuclease recognises specific palindromic nucleotide sequences in the DNA.
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संबंधित प्रश्न
State the role of DNA ligase in biotechnology.
Give the applications of the PCR technique.
Give one significant difference between:
Electroporation and Gene Gun.
Which of the following device is required for PCR?
______ is a Type I restriction enzyme.
Following are the four statements regarding separation of DNA fragments using Gel electrophoresis. Identify the INCORRECT statements.
i. DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal.
ii. DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
iii. Smaller the size of DNA fragment, larger is the distance it travels through it.
iv. Pure DNA can be visualized directly by exposing to UV radiation.
The vector Baculovirus is used for gene transfer in ____________.
What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing?
What is polymerase chain reaction (PCR)?
What is EcoRI?
