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प्रश्न
Which molecules are called molecular scissors? Why are they called so?
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उत्तर
Restriction enzymes are called molecular scissors.
Each restriction endonuclease inspects the length of a DNA sequence. Once it finds its specific recognition sequence, it will bind to the DNA and cut each of the two strands of the double helix at specific points in their sugar-phosphate backbones. Each restriction endonuclease recognises specific palindromic nucleotide sequences in the DNA.
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संबंधित प्रश्न
Identify the CORRECT order of steps in Polymerase Chain Reaction (PCR).
The source of taq Polymerase used in PCR is a ______.
DNA fingerprinting refers to ______.
| Oil spill is a major environmental issue. It has been found that different strains of Pseudomonas bacteria have genes to break down the four major groups of hydrocarbons in oil. Trials are underway to use different biotechnological tools to incorporate these genes and create a genetically engineered strain of Pseudomonas - a ‘super-bug’, to break down the four major groups of hydrocarbons in oil. Such bacteria might be sprayed onto surfaces polluted with oil to clean thin films of oil. |
(a) List two advantages of using bacteria for such biotechnological studies?
(b) For amplification of the gene of interest PCR was carried out. The PCR was run with the help of polymerase which was functional only at a very low temperature. How will this impact the efficiency of the PCR? Justify.
(c) If such bacteria are sprayed on water bodies with oil spills, how will this have a positive or negative effect on the environment? Discuss.
Which of the following is the correct recognition sequence of restriction enzyme Eco RI?
The given figure is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly indentites its certain component(s)?
Which one of the following enzymes is used to join DNA fragments?
David is a molecular biologist. He uses a technique to know the amino acids occupy the first and the last positions in the Chain - A and Chain - B of insulin. Name the scientist whose contribution made it possible for David to use this technique.
The restriction enzymes used as molecular scissors are type of ______.
What is the primary focus of genetic engineering?
