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प्रश्न
What is polymerase chain reaction (PCR)?
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उत्तर १
Polymerase chain reaction (PCR) is another device used for gene cloning or gene multiplication in vitro.
उत्तर २
Polymerase Chain Reaction (PCR) is an in vitro technique used to generate millions of copies of a specific DNA segment rapidly. PCR involves three main steps repeated in cycles: denaturation (heating DNA to separate strands), annealing (binding of primers to target DNA), and extension (DNA polymerase synthesizes new DNA). The enzyme used is Taq polymerase, which is heat-stable and derived from Thermus aquaticus. PCR amplifies DNA to make numerous copies from a small initial sample, facilitating genetic research and diagnostics.
संबंधित प्रश्न
Retroviruses have no DNA. However, the DNA of the infected host cell does possess viral DNA. How is it possible?
The enzyme nuclease hydrolyses ______ of polynucleotide chain of DNA.
Explain the properties of a good or ideal cloning vector for rDNA technology.
Enlist and write in brief about the different biological tools required in rDNA technology.
What is PCR? Explain different steps involved in it.
The time taken to complete one cycle of PCR is around ______.
From the following identify the methods of electrophoresis.
Polymerase Chain Reaction is made possible by ____________.
Name and describe the technique that helps in separation of DNA fragments.
Which molecules are called molecular scissors? Why are they called so?
Which one is a true statement regarding DNA polymerase used in PCR?
Nucleic acid segment tagged with a radioactive molecule is called ______.
The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as ______.
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
Write a short note on vectors.
What is EcoRI?
How does EcoRI differ from an exonuclease?
Importance of PCR.
Assertion: In a bioreactor, it is not necessary to maintain sterile ambience.
Reason: Sterile conditions promote the growth of unwanted microbes in the culture medium.
