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प्रश्न
What is polymerase chain reaction (PCR)?
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उत्तर १
Polymerase chain reaction (PCR) is another device used for gene cloning or gene multiplication in vitro.
उत्तर २
Polymerase Chain Reaction (PCR) is an in vitro technique used to generate millions of copies of a specific DNA segment rapidly. PCR involves three main steps repeated in cycles: denaturation (heating DNA to separate strands), annealing (binding of primers to target DNA), and extension (DNA polymerase synthesizes new DNA). The enzyme used is Taq polymerase, which is heat-stable and derived from Thermus aquaticus. PCR amplifies DNA to make numerous copies from a small initial sample, facilitating genetic research and diagnostics.
संबंधित प्रश्न
Retroviruses have no DNA. However, the DNA of the infected host cell does possess viral DNA. How is it possible?
Distinguish between plasmid DNA and chromosomal DNA.
Give the applications of the PCR technique.
Expand the following abbreviation: BAC
The enzyme restriction endonuclease ______.
For which of the following purpose restriction endonuclease enzyme is used in genetic engineering?
Identify the properties that a cloning vector must possess to be efficiently used in rDNA technology.
- Control elements like promoter, operator, ribosomal binding sites
- Restriction sites
- Marker genes
- Ori of replication
- Ability to replicate independently
Consider there is only one double stranded DNA molecule initially along with all other requirements of PCR. If a PCR machines performs 10 cycles of reaction then bow many double stranded DNA molecules would be present at the end?
Which of the following is the right combination of primers in a PCR to amplify the DNA product?
Study the following statements regarding separation of DNA using agarose gel electrophoresis.
- Large fragments of DNA molecules move faster than small DNA fragments and thus separate faster.
- DNA being negatively charged migrates to cathode.
Following are the four statements regarding separation of DNA fragments using Gel electrophoresis. Identify the INCORRECT statements.
i. DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal.
ii. DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
iii. Smaller the size of DNA fragment, larger is the distance it travels through it.
iv. Pure DNA can be visualized directly by exposing to UV radiation.
The rDNA technology has provided a method to control the nematode parasite Meloidogyne incognita. Explain the principle involved in this technique.
DNA fingerprinting refers to ______.
Expand the following acronym:
BAC
Which of these are most widely used in genetic engineering?
What are the different types of electrophoresis?
Tissue plasminogen activator and urokinase are used in the treatment of ______.
What is EcoRI?
Name the source of thermostable DNA polymerase.
The restriction enzymes used as molecular scissors are type of ______.
