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प्रश्न
What is polymerase chain reaction (PCR)?
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उत्तर १
Polymerase chain reaction (PCR) is another device used for gene cloning or gene multiplication in vitro.
उत्तर २
Polymerase Chain Reaction (PCR) is an in vitro technique used to generate millions of copies of a specific DNA segment rapidly. PCR involves three main steps repeated in cycles: denaturation (heating DNA to separate strands), annealing (binding of primers to target DNA), and extension (DNA polymerase synthesizes new DNA). The enzyme used is Taq polymerase, which is heat-stable and derived from Thermus aquaticus. PCR amplifies DNA to make numerous copies from a small initial sample, facilitating genetic research and diagnostics.
संबंधित प्रश्न
What would be the molar concentration of human DNA in a human cell?
Name and explain the technique used for separating DNA fragments and making them available for biotechnology experiments.
Write a short note on Electrophoresis.
In vitro amplification of DNA or RNA segment is known as ___________.
The recognition sequence of restriction enzymes is generally ______ nucleotide long.
From the following identify the methods of electrophoresis.
Which of the following is an INCORRECT match?
Which of the following is the right combination of primers in a PCR to amplify the DNA product?
Which of the following is NOT involved in genetic engineering technique?
i. Combining genes from two organisms
ii. Gene cloning
iii. Transfer of lipid molecules
iv. Transfer of genes to new organisms
v. Repairing or replacing the defective genes by healthy genes
vi. Artificial synthesis of new gene
Which of the following viruses has the capacity to produce lysozymes?
What is meant by gene cloning?
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
The given figure is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly indentites its certain component(s)?
Study the following lists and choose the correct match.
| List I | List II |
| A. Vector | (i) Resistant to cotton bollworms |
| B. Downstream processing | (ii) Mobile genetic elements |
| C. Cry II AB | (iii) Controls corn borers |
| D. Transposons | (iv) Ti plasmid |
| (v) Purifying protein in biopharmaceuticals |
Describe three steps involved in mechanism of PCR.
______technique is used for in vitro gene cloning or gene multiplication.
Which amino acid is coded by the initiation codon?
Explain the mechanism of PCR.
In vitro amplification of DNA segments is called______.
Give a reason for the following:
DNA cannot enter directly into the host cell.
