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प्रश्न
The rDNA technology has provided a method to control the nematode parasite Meloidogyne incognita. Explain the principle involved in this technique.
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उत्तर
RNA interference (RNAi) is a gene-silencing process that blocks the expression of genes in the parasite when it enters the host's body. This method is used to prevent infestation of roots of tobacco plants by a nematode Meloidogyne incognita. In RNAi, a complementary RNA binds to mRNA to form a dsRNA that cannot translate and hence its expression is blocked. Synthetic neurotransmitants mixed with dsRNA solutions are used for in vitro RNAi in plant-parasitic nematodes with significant success. This introduced DNA which forms both sense and anti-sense RNA. These two strands are complementary to each other bend and form dsRNA leading to RNA interference. mRNA of nematodes is thus silent and the parasite cannot survive in the transgenic host. Thus through this method tobacco plants can be protected from nematode attacks.
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संबंधित प्रश्न
What does PCR stand for? Describe the different steps of PCR
Give the applications of the PCR technique.
Explain the properties of a good or ideal cloning vector for rDNA technology.
Match the List I with List II and List ill. Select the correct option.
| List I | List II | List ill | |||
| i. | Hind III | a. | Agarose ge | 1. | Six base pairs |
| ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marke |
| iii. | T-DNA | c. | Ampicillin | 3. | Elution |
| iv. | DNA | d. | Recognition sequenc | 4. | Transgenic plant |
Match the Column I (Restriction enzyme) with Column II (Recognition sequence) and select the correct option.
| column I (Restriction Enzyme) | column II (Recognition sequence) | ||
| i. | Alu | P. | 5'---G- ↓-G-A-T-C-C---3' 3'---C-C-T-A-G- -G---5 |
| ii. | BamHI | Q. | 5'--- A-G- ↓ -C-T---3' 3' ---T-C- G-A--- 5' |
| iii. | Eco RI | R. | 5'--G-T-C- ↓ -G-A-C--3' I 3'--C-A-G-t-C-T-G--5' |
| iv. | HindII | S. | 5'---G- ↓ -A-A-T-T-C---3' 3'---C-T-T-A-A- -G---5' |
Lytic cycle involves bacteriophage and ____________.
The main reason for the presence of both a leading and a lagging strand during DNA replication is, ______
A hind of biotechnology involving manipulation of DNA is ______.
Which of the following statements are true about cloning vectors?
- Vectors are DNA molecules that carry a foreign DNA segment.
- The replicated DNA is introduced into the host cell.
- It possesses the origin of replication (Ori).
- Vectors may be plasmid, bacteriophage, Yeast artificial chromosomes (YACs), etc.
- Vectors do not require a selectable marker to identify and eliminate non-transformants.
Choose the correct answer from the options given below.
Give a reason for the following:
The diagram given below represents vector pBr322 which was modified by a research scholar Dr. Rayon, but the vector was rejected by his guide.
