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प्रश्न
The rDNA technology has provided a method to control the nematode parasite Meloidogyne incognita. Explain the principle involved in this technique.
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उत्तर
RNA interference (RNAi) is a gene-silencing process that blocks the expression of genes in the parasite when it enters the host's body. This method is used to prevent infestation of roots of tobacco plants by a nematode Meloidogyne incognita. In RNAi, a complementary RNA binds to mRNA to form a dsRNA that cannot translate and hence its expression is blocked. Synthetic neurotransmitants mixed with dsRNA solutions are used for in vitro RNAi in plant-parasitic nematodes with significant success. This introduced DNA which forms both sense and anti-sense RNA. These two strands are complementary to each other bend and form dsRNA leading to RNA interference. mRNA of nematodes is thus silent and the parasite cannot survive in the transgenic host. Thus through this method tobacco plants can be protected from nematode attacks.
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संबंधित प्रश्न
Name and explain the technique used for separating DNA fragments and making them available for biotechnology experiments.
The technique which involves addition or deletion of genes is ______.
The vector Baculovirus is used for gene transfer in ____________.
A hind of biotechnology involving manipulation of DNA is ______.
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?
The PCR technique is used for ______.
Which of the following enzyme catalyses the removal of nucleotides from the end of DNA molecules?
Identify the desirable characteristics for a plasmid used in rDNA technology from the following.
- Ability to multiply and express outside the host in a bioreactor.
- A highly active promoter.
- A site at which replication can be initiated.
- One or more identifiable marker genes.
- One or more unique restriction sites.
The DNA polymerase enzyme used in PCR is obtained from______.
The restriction enzymes used as molecular scissors are type of ______.
