Advertisements
Advertisements
प्रश्न
The rDNA technology has provided a method to control the nematode parasite Meloidogyne incognita. Explain the principle involved in this technique.
Advertisements
उत्तर
RNA interference (RNAi) is a gene-silencing process that blocks the expression of genes in the parasite when it enters the host's body. This method is used to prevent infestation of roots of tobacco plants by a nematode Meloidogyne incognita. In RNAi, a complementary RNA binds to mRNA to form a dsRNA that cannot translate and hence its expression is blocked. Synthetic neurotransmitants mixed with dsRNA solutions are used for in vitro RNAi in plant-parasitic nematodes with significant success. This introduced DNA which forms both sense and anti-sense RNA. These two strands are complementary to each other bend and form dsRNA leading to RNA interference. mRNA of nematodes is thus silent and the parasite cannot survive in the transgenic host. Thus through this method tobacco plants can be protected from nematode attacks.
APPEARS IN
संबंधित प्रश्न
Retroviruses have no DNA. However, the DNA of the infected host cell does possess viral DNA. How is it possible?
Match the Column I (Restriction enzyme) with Column II (Recognition sequence) and select the correct option.
| column I (Restriction Enzyme) | column II (Recognition sequence) | ||
| i. | Alu | P. | 5'---G- ↓-G-A-T-C-C---3' 3'---C-C-T-A-G- -G---5 |
| ii. | BamHI | Q. | 5'--- A-G- ↓ -C-T---3' 3' ---T-C- G-A--- 5' |
| iii. | Eco RI | R. | 5'--G-T-C- ↓ -G-A-C--3' I 3'--C-A-G-t-C-T-G--5' |
| iv. | HindII | S. | 5'---G- ↓ -A-A-T-T-C---3' 3'---C-T-T-A-A- -G---5' |
______ is a Type I restriction enzyme.
The gene transfer between unrelated plants is brought about by ____________.
The main reason for the presence of both a leading and a lagging strand during DNA replication is, ______
Name and describe the technique that helps in separation of DNA fragments.
Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?
Expand the following acronym:
BAC
The polymerase chain reaction is a powerful technique to ______.
Importance of PCR.
