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प्रश्न
Give the applications of the PCR technique.
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उत्तर
PCR is used in:
- DNA cloning.
- Gene amplification.
- DNA-based phylogeny or functional analysis of genes.
- Diagnosis of hereditary disease.
- DNA fingerprinting.
- Diagnosis of infectious diseases and cancer.
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संबंधित प्रश्न
State the role of DNA ligase in biotechnology.
Explain what are the desirable characteristics of an ideal cloning vector used in rDNA technology.
The enzyme nuclease hydrolyses ______ of polynucleotide chain of DNA.
Enlist and write in brief about the different biological tools required in rDNA technology.
Consider there is only one double stranded DNA molecule initially along with all other requirements of PCR. If a PCR machines performs 10 cycles of reaction then bow many double stranded DNA molecules would be present at the end?
Name and describe the technique that helps in separation of DNA fragments.
The source of taq Polymerase used in PCR is a ______.
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
Which vector is mostly used in rDNA technology in plants?
Which of the following statements are true about cloning vectors?
- Vectors are DNA molecules that carry a foreign DNA segment.
- The replicated DNA is introduced into the host cell.
- It possesses the origin of replication (Ori).
- Vectors may be plasmid, bacteriophage, Yeast artificial chromosomes (YACs), etc.
- Vectors do not require a selectable marker to identify and eliminate non-transformants.
Choose the correct answer from the options given below.
