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प्रश्न
Give the applications of the PCR technique.
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उत्तर
PCR is used in:
- DNA cloning.
- Gene amplification.
- DNA-based phylogeny or functional analysis of genes.
- Diagnosis of hereditary disease.
- DNA fingerprinting.
- Diagnosis of infectious diseases and cancer.
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संबंधित प्रश्न
Give an example of the source of thermostable enzyme DNA polymerase.
Explain what are the desirable characteristics of an ideal cloning vector used in rDNA technology.
Ti plasmid being used for introducing genes in plants obtained from ______.
For which of the following purpose restriction endonuclease enzyme is used in genetic engineering?
Identify the characterization techniques that are based on molecular weights of compounds.
In which of the following techniques, charged molecules (DNA, RNA and Proteins) are separated by applying the electric field?
Identify the desirable characteristics for a plasmid used in rDNA technology from the following.
- Ability to multiply and express outside the host in a bioreactor.
- A highly active promoter.
- A site at which replication can be initiated.
- One or more identifiable marker genes.
- One or more unique restriction sites.
Describe three steps involved in mechanism of PCR.
Expand YAC used in the field of Biotechnology.
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
-
Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
