Advertisements
Advertisements
Question
Give the applications of the PCR technique.
Advertisements
Solution
PCR is used in:
- DNA cloning.
- Gene amplification.
- DNA-based phylogeny or functional analysis of genes.
- Diagnosis of hereditary disease.
- DNA fingerprinting.
- Diagnosis of infectious diseases and cancer.
APPEARS IN
RELATED QUESTIONS
What is the role of Taq-polymerase in PCR technology?
Which of the following device is required for PCR?
Which one is a true statement regarding DNA polymerase used in PCR?
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
Expand the following acronym:
BAC
Restriction enzymes were discovered by ______.
What is polymerase chain reaction (PCR)?
Explain the mechanism of PCR.
Give a reason for the following:
The diagram given below represents vector pBr322 which was modified by a research scholar Dr. Rayon, but the vector was rejected by his guide.

