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प्रश्न
The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as ______.
पर्याय
Transduction
Conjugation
Transformation
Translation
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उत्तर
The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as transduction.
APPEARS IN
संबंधित प्रश्न
State the role of DNA ligase in biotechnology.
What are the restriction endonucleases? Give the rules of their nomenclature.
Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
The bacterium which causes a plant disease called crown gall is _______________.
Ti plasmid being used for introducing genes in plants obtained from ______.
Define vector? write any two examples,
Match the List I with List II and List ill. Select the correct option.
| List I | List II | List ill | |||
| i. | Hind III | a. | Agarose ge | 1. | Six base pairs |
| ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marke |
| iii. | T-DNA | c. | Ampicillin | 3. | Elution |
| iv. | DNA | d. | Recognition sequenc | 4. | Transgenic plant |
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
Polymerase Chain Reaction is made possible by ____________.
| Oil spill is a major environmental issue. It has been found that different strains of Pseudomonas bacteria have genes to break down the four major groups of hydrocarbons in oil. Trials are underway to use different biotechnological tools to incorporate these genes and create a genetically engineered strain of Pseudomonas - a ‘super-bug’, to break down the four major groups of hydrocarbons in oil. Such bacteria might be sprayed onto surfaces polluted with oil to clean thin films of oil. |
(a) List two advantages of using bacteria for such biotechnological studies?
(b) For amplification of the gene of interest PCR was carried out. The PCR was run with the help of polymerase which was functional only at a very low temperature. How will this impact the efficiency of the PCR? Justify.
(c) If such bacteria are sprayed on water bodies with oil spills, how will this have a positive or negative effect on the environment? Discuss.
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
What is polymerase chain reaction (PCR)?
Write a short note on vectors.
Name the enzyme responsible for the transcription of genetic code to produce mRNA.
Importance of PCR.
Name the enzymes that are called molecular scissors.
Give a reason for the following:
DNA cannot enter directly into the host cell.
State the steps involved in the process of gene therapy for the treatment of ADA-deficiency.
Suneeta is planning an experiment to clone a gene in a vector. So, she has to choose a good cloning vector.
Which one of the vectors shown below should she choose? Justify your answer by giving two reasons.
 |
 |
 |
| Vector A | Vector B | Vector C |
Give a reason for the following:
The diagram given below represents vector pBr322 which was modified by a research scholar Dr. Rayon, but the vector was rejected by his guide.
