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प्रश्न
One of the major contributions of biotechnology is to develop pest-resistant varieties of cotton plants. Explain how it has been made possible.
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उत्तर
One of the major contributions of biotechnology is the development of insect resistant variety of cotton called Bt cotton. This plant contains the gene (cry gene) from the bacteria Bacillus thuringiensis.
Genes encoded by Cry IAc and Cry II Ab control cotton bollworms and those encoded by Cry IAb control corn borer. The gene from the bacteria is transferred to the plant cell with the help of Agrobacterium Ti-plasmid. The product of this gene is Bt toxin. This toxin is a type of protein that is toxic to a variety of insects including bollworm complex.
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संबंधित प्रश्न
Explain what are the desirable characteristics of an ideal cloning vector used in rDNA technology.
Ti plasmid being used for introducing genes in plants obtained from ______.
Alu-I is obtained from which organism?
Identify the CORRECT sequence of steps in PCR.
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
Which of the following is the correct recognition sequence of restriction enzyme Eco RI?
The given figure is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly indentites its certain component(s)?
Write a short note on vectors.
Explain the mechanism of PCR.
Name the source of thermostable DNA polymerase.
