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प्रश्न
One of the major contributions of biotechnology is to develop pest-resistant varieties of cotton plants. Explain how it has been made possible.
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उत्तर
One of the major contributions of biotechnology is the development of insect resistant variety of cotton called Bt cotton. This plant contains the gene (cry gene) from the bacteria Bacillus thuringiensis.
Genes encoded by Cry IAc and Cry II Ab control cotton bollworms and those encoded by Cry IAb control corn borer. The gene from the bacteria is transferred to the plant cell with the help of Agrobacterium Ti-plasmid. The product of this gene is Bt toxin. This toxin is a type of protein that is toxic to a variety of insects including bollworm complex.
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संबंधित प्रश्न
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| List I | List II | List ill | |||
| i. | Hind III | a. | Agarose ge | 1. | Six base pairs |
| ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marke |
| iii. | T-DNA | c. | Ampicillin | 3. | Elution |
| iv. | DNA | d. | Recognition sequenc | 4. | Transgenic plant |
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Study the diagram given below and answer the questions that follow.

- Name the cloning vector shown above. In which organism in this cloning vector inserted?
-
Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
