Advertisements
Advertisements
Question
One of the major contributions of biotechnology is to develop pest-resistant varieties of cotton plants. Explain how it has been made possible.
Advertisements
Solution
One of the major contributions of biotechnology is the development of insect resistant variety of cotton called Bt cotton. This plant contains the gene (cry gene) from the bacteria Bacillus thuringiensis.
Genes encoded by Cry IAc and Cry II Ab control cotton bollworms and those encoded by Cry IAb control corn borer. The gene from the bacteria is transferred to the plant cell with the help of Agrobacterium Ti-plasmid. The product of this gene is Bt toxin. This toxin is a type of protein that is toxic to a variety of insects including bollworm complex.
APPEARS IN
RELATED QUESTIONS
A PCR machine can raise the temperature up to 100°C but after that, it is not able to lower the temperature below 70°C automatically. Which step of PCR will be hampered first in this faulty machine? Explain why?
What is Palindromic sequence?
What is Recognition sequence? Explain in brief.
From the following list of cloning vectors used in rDNA technology identify the vectors that are the most commonly used.
Plasmids, bacteriophages (Ml3, lambda virus), cosmid, phagemid, BAC (bacterial artificial chromosome), YAC (yeast artificial chromosome), transposons, baculoviruses, and mammalian artificial chromosomes (MACs)
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
Identify the CORRECT sequence of steps in PCR.
A hind of biotechnology involving manipulation of DNA is ______.
Who among the following was awarded the Nobel Prize for the development of PCR technique?
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
Which of the following statements are true about cloning vectors?
- Vectors are DNA molecules that carry a foreign DNA segment.
- The replicated DNA is introduced into the host cell.
- It possesses the origin of replication (Ori).
- Vectors may be plasmid, bacteriophage, Yeast artificial chromosomes (YACs), etc.
- Vectors do not require a selectable marker to identify and eliminate non-transformants.
Choose the correct answer from the options given below.
