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प्रश्न
One of the major contributions of biotechnology is to develop pest-resistant varieties of cotton plants. Explain how it has been made possible.
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उत्तर
One of the major contributions of biotechnology is the development of insect resistant variety of cotton called Bt cotton. This plant contains the gene (cry gene) from the bacteria Bacillus thuringiensis.
Genes encoded by Cry IAc and Cry II Ab control cotton bollworms and those encoded by Cry IAb control corn borer. The gene from the bacteria is transferred to the plant cell with the help of Agrobacterium Ti-plasmid. The product of this gene is Bt toxin. This toxin is a type of protein that is toxic to a variety of insects including bollworm complex.
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संबंधित प्रश्न
A PCR machine can raise the temperature up to 100°C but after that, it is not able to lower the temperature below 70°C automatically. Which step of PCR will be hampered first in this faulty machine? Explain why?
Enlist and write in brief about the different biological tools required in rDNA technology.
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
Identify the properties that a cloning vector must possess to be efficiently used in rDNA technology.
- Control elements like promoter, operator, ribosomal binding sites
- Restriction sites
- Marker genes
- Ori of replication
- Ability to replicate independently
Which one is a true statement regarding DNA polymerase used in PCR?
The source of taq Polymerase used in PCR is a ______.
Which of the following is not required in the preparation of a recombinant DNA molecule?
Expand the following acronym:
BAC
Explain the mechanism of PCR.
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