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प्रश्न
One of the major contributions of biotechnology is to develop pest-resistant varieties of cotton plants. Explain how it has been made possible.
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उत्तर
One of the major contributions of biotechnology is the development of insect resistant variety of cotton called Bt cotton. This plant contains the gene (cry gene) from the bacteria Bacillus thuringiensis.
Genes encoded by Cry IAc and Cry II Ab control cotton bollworms and those encoded by Cry IAb control corn borer. The gene from the bacteria is transferred to the plant cell with the help of Agrobacterium Ti-plasmid. The product of this gene is Bt toxin. This toxin is a type of protein that is toxic to a variety of insects including bollworm complex.
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संबंधित प्रश्न
State the role of DNA ligase in biotechnology.
Explain what are the desirable characteristics of an ideal cloning vector used in rDNA technology.
The bacterium which causes a plant disease called crown gall is _______________.
Enlist different types of restriction enzymes commonly used in rDNA technology? Write about their role.
____________ is the manipulation of genetic material towards a desired end, in a directed and predetermined way, using in vitro process.
In PCR technique, the organism providing thermostable DNA polymerase is a ____________.
Identify the desirable characteristics for a plasmid used in rDNA technology from the following.
- Ability to multiply and express outside the host in a bioreactor.
- A highly active promoter.
- A site at which replication can be initiated.
- One or more identifiable marker genes.
- One or more unique restriction sites.
Give a reason for the following:
DNA cannot enter directly into the host cell.
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
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Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
The manipulation of genetic material towards a desired end using "in-vitro" process is called______.
