Advertisements
Advertisements
प्रश्न
The recognition sequence of restriction enzymes is generally ______ nucleotide long.
विकल्प
2 to 4
4 to 8
8 to 10
14 to 18
Advertisements
उत्तर
The recognition sequence of restriction enzymes is generally 4 to 8 nucleotides long.
Explanation:
The sequences recognized by restriction enzymes are 4 to 8 nucleotides long and characterized by a particular type of internal symmetry. Consider the specific sequence recognized by the enzyme EcoRI.
संबंधित प्रश्न
Retroviruses have no DNA. However, the DNA of the infected host cell does possess viral DNA. How is it possible?
The biological scissor is ______.
What are the restriction endonucleases? Give the rules of their nomenclature.
Describe two vectors less methods of gene transfer used in rDNA technology.
What is gene cloning? Explain different tools used for it.
The time taken to complete one cycle of PCR is around ______.
The gene transfer between unrelated plants is brought about by ____________.
What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing?
| Oil spill is a major environmental issue. It has been found that different strains of Pseudomonas bacteria have genes to break down the four major groups of hydrocarbons in oil. Trials are underway to use different biotechnological tools to incorporate these genes and create a genetically engineered strain of Pseudomonas - a ‘super-bug’, to break down the four major groups of hydrocarbons in oil. Such bacteria might be sprayed onto surfaces polluted with oil to clean thin films of oil. |
(a) List two advantages of using bacteria for such biotechnological studies?
(b) For amplification of the gene of interest PCR was carried out. The PCR was run with the help of polymerase which was functional only at a very low temperature. How will this impact the efficiency of the PCR? Justify.
(c) If such bacteria are sprayed on water bodies with oil spills, how will this have a positive or negative effect on the environment? Discuss.
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
-
Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
