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प्रश्न
What is plasmid?
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उत्तर १
Plasmid is a circular, double stranded, self-replicating, extra-chromosomal DNA molecule.
उत्तर २
Plasmids are small, circular and duplex DNA molecules and their natural function is to confer antibiotic resistance to the host cell. They have several properties that make them extremely useful as cloning vectors.
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संबंधित प्रश्न
Expand the following abbreviation: BAC
Explain what are the desirable characteristics of an ideal cloning vector used in rDNA technology.
Describe two vectors less methods of gene transfer used in rDNA technology.
The enzyme nuclease hydrolyses ______ of polynucleotide chain of DNA.
Ti plasmid being used for introducing genes in plants obtained from ______.
From the following which one is NOT a feature of the plasmids?
Identify the properties that a cloning vector must possess to be efficiently used in rDNA technology.
- Control elements like promoter, operator, ribosomal binding sites
- Restriction sites
- Marker genes
- Ori of replication
- Ability to replicate independently
Which of the following DNA sequence is recognised and cut by EcoR I?
Which of the following is the right combination of primers in a PCR to amplify the DNA product?
Name the plant disease caused by Agrobacterium tumefaciens.
The PCR technique is used for ______.
Which amino acid is coded by the initiation codon?
Which one of the following enzymes is used to join DNA fragments?
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
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Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
Suneeta is planning an experiment to clone a gene in a vector. So, she has to choose a good cloning vector.
Which one of the vectors shown below should she choose? Justify your answer by giving two reasons.
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 |
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| Vector A | Vector B | Vector C |
The manipulation of genetic material towards a desired end using "in-vitro" process is called______.
What is the relationship between alien DNA and the origin of replication in the concept of cloning?
What is the function of DNA ligase in the creation of recombinant DNA?
The origin of replication is best described as:
