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प्रश्न
What is plasmid?
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उत्तर १
Plasmid is a circular, double stranded, self-replicating, extra-chromosomal DNA molecule.
उत्तर २
Plasmids are small, circular and duplex DNA molecules and their natural function is to confer antibiotic resistance to the host cell. They have several properties that make them extremely useful as cloning vectors.
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संबंधित प्रश्न
What does PCR stand for? Describe the different steps of PCR
A PCR machine can raise the temperature up to 100°C but after that, it is not able to lower the temperature below 70°C automatically. Which step of PCR will be hampered first in this faulty machine? Explain why?
Give the steps in PCR or polymerase chain reaction with suitable diagrams.
The enzyme restriction endonuclease ______.
Polymerase chain reaction is most useful in ______.
What is the role of Taq-polymerase in PCR technology?
From the following list of cloning vectors used in rDNA technology identify the vectors that are the most commonly used.
Plasmids, bacteriophages (Ml3, lambda virus), cosmid, phagemid, BAC (bacterial artificial chromosome), YAC (yeast artificial chromosome), transposons, baculoviruses, and mammalian artificial chromosomes (MACs)
Which of the following device is required for PCR?
In PCR technique, the organism providing thermostable DNA polymerase is a ____________.
The gene transfer between unrelated plants is brought about by ____________.
Which of the following viruses has the capacity to produce lysozymes?
A hind of biotechnology involving manipulation of DNA is ______.
Name a recombinant vaccine that is currently being used in vaccination programme.
Name two restriction enzymes used in PCR.
pBR322, used very frequently in cloning experiments as a vector in E. coli is ______.
The DNA polymerase enzyme used in PCR is obtained from______.
Name the enzyme responsible for the transcription of genetic code to produce mRNA.
Importance of PCR.
How many sets of primers are required in each cycle of PCR?
Suneeta is planning an experiment to clone a gene in a vector. So, she has to choose a good cloning vector.
Which one of the vectors shown below should she choose? Justify your answer by giving two reasons.
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| Vector A | Vector B | Vector C |
