Advertisements
Advertisements
प्रश्न
What is plasmid?
Advertisements
उत्तर १
Plasmid is a circular, double stranded, self-replicating, extra-chromosomal DNA molecule.
उत्तर २
Plasmids are small, circular and duplex DNA molecules and their natural function is to confer antibiotic resistance to the host cell. They have several properties that make them extremely useful as cloning vectors.
APPEARS IN
संबंधित प्रश्न
How has the development of bioreactor helped in biotechnology?
Give the applications of the PCR technique.
Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
The recognition sequence of restriction enzymes is generally ______ nucleotide long.
Vectors are ____________ molecules that carry a foreign gene and replicate inside the host cell.
Identify the CORRECT sequence of steps in PCR.
For which of the following purpose restriction endonuclease enzyme is used in genetic engineering?
Which of the following is an INCORRECT match?
Which of the following DNA sequence is recognised and cut by EcoR I?
______ is a Type I restriction enzyme.
The gene transfer between unrelated plants is brought about by ____________.
Name and describe the technique that helps in separation of DNA fragments.
What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing?
| Oil spill is a major environmental issue. It has been found that different strains of Pseudomonas bacteria have genes to break down the four major groups of hydrocarbons in oil. Trials are underway to use different biotechnological tools to incorporate these genes and create a genetically engineered strain of Pseudomonas - a ‘super-bug’, to break down the four major groups of hydrocarbons in oil. Such bacteria might be sprayed onto surfaces polluted with oil to clean thin films of oil. |
(a) List two advantages of using bacteria for such biotechnological studies?
(b) For amplification of the gene of interest PCR was carried out. The PCR was run with the help of polymerase which was functional only at a very low temperature. How will this impact the efficiency of the PCR? Justify.
(c) If such bacteria are sprayed on water bodies with oil spills, how will this have a positive or negative effect on the environment? Discuss.
Which of these are most widely used in genetic engineering?
To be a cloning vector, a plasmid require ______.
The polymerase chain reaction is a powerful technique to ______.
How many sets of primers are required in each cycle of PCR?
What is the primary focus of genetic engineering?
Genetic engineering is also known as:
