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प्रश्न
What is plasmid?
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उत्तर १
Plasmid is a circular, double stranded, self-replicating, extra-chromosomal DNA molecule.
उत्तर २
Plasmids are small, circular and duplex DNA molecules and their natural function is to confer antibiotic resistance to the host cell. They have several properties that make them extremely useful as cloning vectors.
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संबंधित प्रश्न
Give an account of the Blue-White Method of selection of recombinants.
How has the development of bioreactor helped in biotechnology?
Give the steps in PCR or polymerase chain reaction with suitable diagrams.
What is gene cloning? Explain different tools used for it.
Identify the CORRECT sequence of steps in PCR.
The time taken to complete one cycle of PCR is around ______.
Match the column I (PCR steps) and Column II (Temperature). Select the correct option.
| Column I (PCR steps) | Column II (Temperature) | ||
| i. | Polymerization | P. | 40 - 60°C |
| ii. | Denaturation | Q. | 70 - 75°C |
| iii. | Primer annealing | R. | 90 - 98°C |
| S. | 28 - 37°C |
Lytic cycle involves bacteriophage and ____________.
A technique used to make numerous copies of a specific DNA segment quickly and accurately is called ______
Which of the following statements is correct with respect to cellular respiration?
Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?
Expand the following acronym:
BAC
The given figure is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly indentites its certain component(s)?
Plasmid is ______.
How gene amplification is done?
______technique is used for in vitro gene cloning or gene multiplication.
Name the enzyme responsible for the transcription of genetic code to produce mRNA.
David is a molecular biologist. He uses a technique to know the amino acids occupy the first and the last positions in the Chain - A and Chain - B of insulin. Name the scientist whose contribution made it possible for David to use this technique.
Give a reason for the following:
The diagram given below represents vector pBr322 which was modified by a research scholar Dr. Rayon, but the vector was rejected by his guide.
