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प्रश्न
What is plasmid?
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उत्तर १
Plasmid is a circular, double stranded, self-replicating, extra-chromosomal DNA molecule.
उत्तर २
Plasmids are small, circular and duplex DNA molecules and their natural function is to confer antibiotic resistance to the host cell. They have several properties that make them extremely useful as cloning vectors.
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संबंधित प्रश्न
Give one significant difference between:
Electroporation and Gene Gun.
Enlist and write in brief about the different biological tools required in rDNA technology.
The technique which involves addition or deletion of genes is ______.
DNA polymerase enzyme used in PCR is isolated from ____________ bacteria.
From the following identify the methods of electrophoresis.
Study the following statements regarding separation of DNA using agarose gel electrophoresis.
- Large fragments of DNA molecules move faster than small DNA fragments and thus separate faster.
- DNA being negatively charged migrates to cathode.
____________ is the manipulation of genetic material towards a desired end, in a directed and predetermined way, using in vitro process.
The vector Baculovirus is used for gene transfer in ____________.
Exonuclease performs the function of ____________.
Nucleic acid segment tagged with a radioactive molecule is called ______.
The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as ______.
Annealing step of PCR operates at ______ °C.
Which of the following is the correct recognition sequence of restriction enzyme Eco RI?
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
Plasmid is ______.
______technique is used for in vitro gene cloning or gene multiplication.
What are the different types of electrophoresis?
Which one of the following enzymes is used to join DNA fragments?
David is a molecular biologist. He uses a technique to know the amino acids occupy the first and the last positions in the Chain - A and Chain - B of insulin. Name the scientist whose contribution made it possible for David to use this technique.
The restriction enzymes used as molecular scissors are type of ______.
