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प्रश्न
What is plasmid?
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उत्तर १
Plasmid is a circular, double stranded, self-replicating, extra-chromosomal DNA molecule.
उत्तर २
Plasmids are small, circular and duplex DNA molecules and their natural function is to confer antibiotic resistance to the host cell. They have several properties that make them extremely useful as cloning vectors.
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संबंधित प्रश्न
What are plasmids?
The biological scissor is ______.
What is the cell that receives a recombinant gene called?
Give the steps in PCR or polymerase chain reaction with suitable diagrams.
Enlist and write in brief about the different biological tools required in rDNA technology.
Which of the following is TRUE regarding EcoR I?
Match the List I with List II and List ill. Select the correct option.
| List I | List II | List ill | |||
| i. | Hind III | a. | Agarose ge | 1. | Six base pairs |
| ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marke |
| iii. | T-DNA | c. | Ampicillin | 3. | Elution |
| iv. | DNA | d. | Recognition sequenc | 4. | Transgenic plant |
Identify the properties that a cloning vector must possess to be efficiently used in rDNA technology.
- Control elements like promoter, operator, ribosomal binding sites
- Restriction sites
- Marker genes
- Ori of replication
- Ability to replicate independently
Identify the process by which bacteria protect their own DNA from the attack of restriction enzymes.
From which of the following organisms the enzyme Taq polymerase used in PCR technique is obtained?
Which of the following is NOT involved in genetic engineering technique?
i. Combining genes from two organisms
ii. Gene cloning
iii. Transfer of lipid molecules
iv. Transfer of genes to new organisms
v. Repairing or replacing the defective genes by healthy genes
vi. Artificial synthesis of new gene
The source of taq Polymerase used in PCR is a ______.
Annealing step of PCR operates at ______ °C.
Name the plant disease caused by Agrobacterium tumefaciens.
Which of these are most widely used in genetic engineering?
The polymerase chain reaction is a powerful technique to ______.
What are the different types of electrophoresis?
What is EcoRI?
Explain the mechanism of PCR.
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
-
Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
