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प्रश्न
What is plasmid?
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उत्तर १
Plasmid is a circular, double stranded, self-replicating, extra-chromosomal DNA molecule.
उत्तर २
Plasmids are small, circular and duplex DNA molecules and their natural function is to confer antibiotic resistance to the host cell. They have several properties that make them extremely useful as cloning vectors.
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संबंधित प्रश्न
Distinguish between plasmid DNA and chromosomal DNA.
What does PCR stand for? Describe the different steps of PCR
Explain what are the desirable characteristics of an ideal cloning vector used in rDNA technology.
What is the cell that receives a recombinant gene called?
In vitro amplification of DNA or RNA segment is known as ___________.
Enlist different types of restriction enzymes commonly used in rDNA technology? Write about their role.
ECoRI is obtained from ______.
Identify the CORRECT sequence of steps in PCR.
In which of the following techniques, charged molecules (DNA, RNA and Proteins) are separated by applying the electric field?
Which of the following is the right combination of primers in a PCR to amplify the DNA product?
Study the following statements regarding separation of DNA using agarose gel electrophoresis.
- Large fragments of DNA molecules move faster than small DNA fragments and thus separate faster.
- DNA being negatively charged migrates to cathode.
Name the plant disease caused by Agrobacterium tumefaciens.
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
Expand the following acronym:
BAC
The PCR technique is used for ______.
Study the following lists and choose the correct match.
| List I | List II |
| A. Vector | (i) Resistant to cotton bollworms |
| B. Downstream processing | (ii) Mobile genetic elements |
| C. Cry II AB | (iii) Controls corn borers |
| D. Transposons | (iv) Ti plasmid |
| (v) Purifying protein in biopharmaceuticals |
The plasmid of Agrobacterium tumefaciens that is now modified as a cloning vector is ______.
The polymerase chain reaction is a powerful technique to ______.
Tissue plasminogen activator and urokinase are used in the treatment of ______.
In vitro amplification of DNA segments is called______.
