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प्रश्न
Suneeta is planning an experiment to clone a gene in a vector. So, she has to choose a good cloning vector.
Which one of the vectors shown below should she choose? Justify your answer by giving two reasons.
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| Vector A | Vector B | Vector C |
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उत्तर
A cloning vector should have a replication origin that allows it to reproduce itself within the host cell. It should include a restriction site for the insertion of the target DNA. It should include a selectable marker with an antibiotic resistance gene to aid in the screening of the recombinant organism. By taking into account all of the above, She should select Vector-C.
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संबंधित प्रश्न
Enlist different types of restriction enzymes commonly used in rDNA technology? Write about their role.
What is plasmid?
Bt-cotton shows adverse effect on the population of which butterfly?
Identify the characterization techniques that are based on molecular weights of compounds.
Which of the following is NOT involved in genetic engineering technique?
i. Combining genes from two organisms
ii. Gene cloning
iii. Transfer of lipid molecules
iv. Transfer of genes to new organisms
v. Repairing or replacing the defective genes by healthy genes
vi. Artificial synthesis of new gene
Identify the CORRECT order of steps in Polymerase Chain Reaction (PCR).
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
Write steps involved in gene amplification.
Name the source of thermostable DNA polymerase.
Give a reason for the following:
DNA cannot enter directly into the host cell.
