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प्रश्न
Suneeta is planning an experiment to clone a gene in a vector. So, she has to choose a good cloning vector.
Which one of the vectors shown below should she choose? Justify your answer by giving two reasons.
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| Vector A | Vector B | Vector C |
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उत्तर
A cloning vector should have a replication origin that allows it to reproduce itself within the host cell. It should include a restriction site for the insertion of the target DNA. It should include a selectable marker with an antibiotic resistance gene to aid in the screening of the recombinant organism. By taking into account all of the above, She should select Vector-C.
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संबंधित प्रश्न
Distinguish between plasmid DNA and chromosomal DNA.
Expand the following abbreviation: BAC
Explain what are the desirable characteristics of an ideal cloning vector used in rDNA technology.
Why are bio-fertilizers preferred over chemical fertilizers?
Enlist and write in brief about the different biological tools required in rDNA technology.
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
Which of the following is the right combination of primers in a PCR to amplify the DNA product?
____________ is the manipulation of genetic material towards a desired end, in a directed and predetermined way, using in vitro process.
A technique used to make numerous copies of a specific DNA segment quickly and accurately is called ______
State the steps involved in the process of gene therapy for the treatment of ADA-deficiency.
