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प्रश्न
Suneeta is planning an experiment to clone a gene in a vector. So, she has to choose a good cloning vector.
Which one of the vectors shown below should she choose? Justify your answer by giving two reasons.
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| Vector A | Vector B | Vector C |
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उत्तर
A cloning vector should have a replication origin that allows it to reproduce itself within the host cell. It should include a restriction site for the insertion of the target DNA. It should include a selectable marker with an antibiotic resistance gene to aid in the screening of the recombinant organism. By taking into account all of the above, She should select Vector-C.
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संबंधित प्रश्न
Write a short note on Electrophoresis.
The technique which involves addition or deletion of genes is ______.
Ti plasmid being used for introducing genes in plants obtained from ______.
Identify the palindromic sequence which is recognized by the EcoRI restriction enzyme.
Which of the following is the right combination of primers in a PCR to amplify the DNA product?
Lytic cycle involves bacteriophage and ____________.
Which of the following is not required in the preparation of a recombinant DNA molecule?
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
The PCR technique is used for ______.
What is the relationship between alien DNA and the origin of replication in the concept of cloning?
