Advertisements
Advertisements
Question
Suneeta is planning an experiment to clone a gene in a vector. So, she has to choose a good cloning vector.
Which one of the vectors shown below should she choose? Justify your answer by giving two reasons.
 |
 |
 |
| Vector A | Vector B | Vector C |
Advertisements
Solution
A cloning vector should have a replication origin that allows it to reproduce itself within the host cell. It should include a restriction site for the insertion of the target DNA. It should include a selectable marker with an antibiotic resistance gene to aid in the screening of the recombinant organism. By taking into account all of the above, She should select Vector-C.
APPEARS IN
RELATED QUESTIONS
The technique which involves addition or deletion of genes is ______.
Explain types of enzymes used in biotechnology?
What is PCR? Explain different steps involved in it.
Identify the characterization techniques that are based on molecular weights of compounds.
From the following identify the methods of electrophoresis.
What is the significance of adding proteases at the time of isolation of genetic material (DNA)?
Which of these are most widely used in genetic engineering?
How gene amplification is done?
Write a short note on vectors.
Name the enzymes that are called molecular scissors.
