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प्रश्न
Suneeta is planning an experiment to clone a gene in a vector. So, she has to choose a good cloning vector.
Which one of the vectors shown below should she choose? Justify your answer by giving two reasons.
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| Vector A | Vector B | Vector C |
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उत्तर
A cloning vector should have a replication origin that allows it to reproduce itself within the host cell. It should include a restriction site for the insertion of the target DNA. It should include a selectable marker with an antibiotic resistance gene to aid in the screening of the recombinant organism. By taking into account all of the above, She should select Vector-C.
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संबंधित प्रश्न
Distinguish between RNA and DNA.
How has the development of bioreactor helped in biotechnology?
Explain what are the desirable characteristics of an ideal cloning vector used in rDNA technology.
Give the steps in PCR or polymerase chain reaction with suitable diagrams.
Which of the following is an INCORRECT match?
From which of the following organisms the enzyme Taq polymerase used in PCR technique is obtained?
______ is a Type I restriction enzyme.
Which of the following is not required in the preparation of a recombinant DNA molecule?
Which of these are most widely used in genetic engineering?
What are the different types of electrophoresis?
