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Question
What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing?
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Solution
In the absence of antibiotic, there will be no pressure on recombinants to retain the plasmid (containing the gene of your interest). Since, maintaining a high copy number of plasmids is a metabolic burden to the microbial cells, will thus tend to lose the plasmid.
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