Advertisements
Advertisements
Question
What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing?
Advertisements
Solution
In the absence of antibiotic, there will be no pressure on recombinants to retain the plasmid (containing the gene of your interest). Since, maintaining a high copy number of plasmids is a metabolic burden to the microbial cells, will thus tend to lose the plasmid.
APPEARS IN
RELATED QUESTIONS
What does PCR stand for? Describe the different steps of PCR
One of the major contributions of biotechnology is to develop pest-resistant varieties of cotton plants. Explain how it has been made possible.
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
Describe two vectors less methods of gene transfer used in rDNA technology.
Explain the properties of a good or ideal cloning vector for rDNA technology.
Alu-I is obtained from which organism?
What is Recognition sequence? Explain in brief.
DNA polymerase enzyme used in PCR is isolated from ____________ bacteria.
Identify the process by which bacteria protect their own DNA from the attack of restriction enzymes.
Which of the following is the right combination of primers in a PCR to amplify the DNA product?
Study the following statements regarding separation of DNA using agarose gel electrophoresis.
- Large fragments of DNA molecules move faster than small DNA fragments and thus separate faster.
- DNA being negatively charged migrates to cathode.
Identify the CORRECT order of steps in Polymerase Chain Reaction (PCR).
Polymerase Chain Reaction is made possible by ____________.
In PCR technique, the organism providing thermostable DNA polymerase is a ____________.
Human insulin is produced by culturing the genetically engineered ______
DNA fingerprinting refers to ______.
The given figure is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly indentites its certain component(s)?
Describe three steps involved in mechanism of PCR.
How gene amplification is done?
Importance of PCR.
