Question

Study the diagram given below and answer the questions that follow.

1. Name the cloning vector shown above. In which organism in this cloning vector inserted?

2. Mention any two restriction sites shown in the diagram.

3. Name any two selectable markers shown in the diagram.

Answer 1

1. The given cloning vector is called pBR322 and it is inserted into E. coli bacteria.
2. In the image shown, the two restriction sites are Hind III and Bam H I.
3. The selectable markers in the cloning vector pBR322 include ampicillin and tetracycline resistance genes.