Advertisements
Advertisements
Question
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
-
Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
Advertisements
Solution
- The given cloning vector is called pBR322 and it is inserted into E. coli bacteria.
- In the image shown, the two restriction sites are Hind III and Bam H I.
- The selectable markers in the cloning vector pBR322 include ampicillin and tetracycline resistance genes.
APPEARS IN
RELATED QUESTIONS
Retroviruses have no DNA. However, the DNA of the infected host cell does possess viral DNA. How is it possible?
From the following which one is NOT a feature of the plasmids?
The following identifies the major features of technology that differentiate modem biotechnology from classical biotechnology.
i. Capability of science to change the genetic material for getting new specific products through rDNA technology, polymerase chain reaction (PCR), microarrays, cell culture and fusion, and bioprocessing.
ii. Ownership of technology and its sociopolitical impact.
iii. Modem biotechnology makes use of only fermentation technology.
iv. Classical biotechnology relies only on principles of plant and animal tissue culture.
____________ cuts the DNA within the specific positions.
______ is a Type I restriction enzyme.
A technique used to make numerous copies of a specific DNA segment quickly and accurately is called ______
Annealing step of PCR operates at ______ °C.
______technique is used for in vitro gene cloning or gene multiplication.
Name the source of thermostable DNA polymerase.
Assertion: In a bioreactor, it is not necessary to maintain sterile ambience.
Reason: Sterile conditions promote the growth of unwanted microbes in the culture medium.
