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प्रश्न
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
-
Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
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उत्तर
- The given cloning vector is called pBR322 and it is inserted into E. coli bacteria.
- In the image shown, the two restriction sites are Hind III and Bam H I.
- The selectable markers in the cloning vector pBR322 include ampicillin and tetracycline resistance genes.
संबंधित प्रश्न
Name the most commonly used bioreactor and describe its working.
The biological scissor is ______.
From the following list of cloning vectors used in rDNA technology identify the vectors that are the most commonly used.
Plasmids, bacteriophages (Ml3, lambda virus), cosmid, phagemid, BAC (bacterial artificial chromosome), YAC (yeast artificial chromosome), transposons, baculoviruses, and mammalian artificial chromosomes (MACs)
What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing?
Which of the following is the correct recognition sequence of restriction enzyme Eco RI?
Identify the desirable characteristics for a plasmid used in rDNA technology from the following.
- Ability to multiply and express outside the host in a bioreactor.
- A highly active promoter.
- A site at which replication can be initiated.
- One or more identifiable marker genes.
- One or more unique restriction sites.
How many sets of primers are required in each cycle of PCR?
The restriction enzymes used as molecular scissors are type of ______.
What is the function of DNA ligase in the creation of recombinant DNA?
How does the concept of cloning relate to the multiplication of genetic material in biotechnology?
