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प्रश्न
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
-
Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
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उत्तर
- The given cloning vector is called pBR322 and it is inserted into E. coli bacteria.
- In the image shown, the two restriction sites are Hind III and Bam H I.
- The selectable markers in the cloning vector pBR322 include ampicillin and tetracycline resistance genes.
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संबंधित प्रश्न
What are plasmids?
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
Define vector? write any two examples,
For which of the following purpose restriction endonuclease enzyme is used in genetic engineering?
Identify the properties that a cloning vector must possess to be efficiently used in rDNA technology.
- Control elements like promoter, operator, ribosomal binding sites
- Restriction sites
- Marker genes
- Ori of replication
- Ability to replicate independently
In PCR technique, the organism providing thermostable DNA polymerase is a ____________.
A hind of biotechnology involving manipulation of DNA is ______.
Which of the following is not required in the preparation of a recombinant DNA molecule?
Expand the following acronym:
BAC
Which of the following enzyme catalyses the removal of nucleotides from the end of DNA molecules?
