Advertisements
Advertisements
प्रश्न
State the steps involved in the process of gene therapy for the treatment of ADA-deficiency.
Advertisements
उत्तर
- Stem cells from the bone marrow, responsible for producing lymphocytes in ADA-deficient patients, are extracted. These cells harbor the faulty ADA gene.
- A retrovirus, a specialized RNA virus, serves as a vector in this process.
- The normal ADA gene is replicated through recombinant DNA technology and incorporated into the nucleic acid of a retrovirus, engineered to be non-pathogenic.
- The bone marrow stem cells from ADA-deficient individuals undergo infection with the retrovirus.
- The virus introduces its nucleic acid (rDNA), which includes the functional ADA gene, into the chromosome of the stem cell.
- This provides these bone marrow stem cells with a functional copy of the gene alongside the pre-existing defective gene.
- The treated stem cells continue to replicate over the patient's lifetime. Possessing a fully functional ADA gene, they produce the ADA enzyme. These modified cells supply the lacking enzyme, leading to the cure of the ADA-deficient patient.
संबंधित प्रश्न
Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
What is Recognition sequence? Explain in brief.
Identify the characterization techniques that are based on molecular weights of compounds.
Match the Column I (Restriction enzyme) with Column II (Recognition sequence) and select the correct option.
| column I (Restriction Enzyme) | column II (Recognition sequence) | ||
| i. | Alu | P. | 5'---G- ↓-G-A-T-C-C---3' 3'---C-C-T-A-G- -G---5 |
| ii. | BamHI | Q. | 5'--- A-G- ↓ -C-T---3' 3' ---T-C- G-A--- 5' |
| iii. | Eco RI | R. | 5'--G-T-C- ↓ -G-A-C--3' I 3'--C-A-G-t-C-T-G--5' |
| iv. | HindII | S. | 5'---G- ↓ -A-A-T-T-C---3' 3'---C-T-T-A-A- -G---5' |
Which of the following DNA sequence is recognised and cut by EcoR I?
Which of the following viruses has the capacity to produce lysozymes?
The source of taq Polymerase used in PCR is a ______.
Who among the following was awarded the Nobel Prize for the development of PCR technique?
Which of the following statements are true about cloning vectors?
- Vectors are DNA molecules that carry a foreign DNA segment.
- The replicated DNA is introduced into the host cell.
- It possesses the origin of replication (Ori).
- Vectors may be plasmid, bacteriophage, Yeast artificial chromosomes (YACs), etc.
- Vectors do not require a selectable marker to identify and eliminate non-transformants.
Choose the correct answer from the options given below.
Describe three steps involved in mechanism of PCR.
