Advertisements
Advertisements
प्रश्न
State the steps involved in the process of gene therapy for the treatment of ADA-deficiency.
Advertisements
उत्तर
- Stem cells from the bone marrow, responsible for producing lymphocytes in ADA-deficient patients, are extracted. These cells harbor the faulty ADA gene.
- A retrovirus, a specialized RNA virus, serves as a vector in this process.
- The normal ADA gene is replicated through recombinant DNA technology and incorporated into the nucleic acid of a retrovirus, engineered to be non-pathogenic.
- The bone marrow stem cells from ADA-deficient individuals undergo infection with the retrovirus.
- The virus introduces its nucleic acid (rDNA), which includes the functional ADA gene, into the chromosome of the stem cell.
- This provides these bone marrow stem cells with a functional copy of the gene alongside the pre-existing defective gene.
- The treated stem cells continue to replicate over the patient's lifetime. Possessing a fully functional ADA gene, they produce the ADA enzyme. These modified cells supply the lacking enzyme, leading to the cure of the ADA-deficient patient.
APPEARS IN
संबंधित प्रश्न
The enzyme restriction endonuclease ______.
For which of the following reason Agrobacterium tumefaciens is most widely used for gene transfer in plants?
Consider there is only one double stranded DNA molecule initially along with all other requirements of PCR. If a PCR machines performs 10 cycles of reaction then bow many double stranded DNA molecules would be present at the end?
| Oil spill is a major environmental issue. It has been found that different strains of Pseudomonas bacteria have genes to break down the four major groups of hydrocarbons in oil. Trials are underway to use different biotechnological tools to incorporate these genes and create a genetically engineered strain of Pseudomonas - a ‘super-bug’, to break down the four major groups of hydrocarbons in oil. Such bacteria might be sprayed onto surfaces polluted with oil to clean thin films of oil. |
(a) List two advantages of using bacteria for such biotechnological studies?
(b) For amplification of the gene of interest PCR was carried out. The PCR was run with the help of polymerase which was functional only at a very low temperature. How will this impact the efficiency of the PCR? Justify.
(c) If such bacteria are sprayed on water bodies with oil spills, how will this have a positive or negative effect on the environment? Discuss.
Which vector is mostly used in rDNA technology in plants?
Plasmid is ______.
What are the different types of electrophoresis?
In vitro amplification of DNA segments is called______.
Give a reason for the following:
DNA cannot enter directly into the host cell.
What is the function of DNA ligase in the creation of recombinant DNA?
