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प्रश्न
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
-
Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
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उत्तर
- The given cloning vector is called pBR322 and it is inserted into E. coli bacteria.
- In the image shown, the two restriction sites are Hind III and Bam H I.
- The selectable markers in the cloning vector pBR322 include ampicillin and tetracycline resistance genes.
संबंधित प्रश्न
Explain the work carried out by Cohen and Boyer that contributed immensely in biotechnology.
The enzyme restriction endonuclease ______.
Identify the palindromic sequence which is recognized by the EcoRI restriction enzyme.
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
A technique used to make numerous copies of a specific DNA segment quickly and accurately is called ______
The source of taq Polymerase used in PCR is a ______.
What is true for plasmid?
Which of these are most widely used in genetic engineering?
How many sets of primers are required in each cycle of PCR?
Genetic engineering is also known as:
