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प्रश्न
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
-
Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
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उत्तर
- The given cloning vector is called pBR322 and it is inserted into E. coli bacteria.
- In the image shown, the two restriction sites are Hind III and Bam H I.
- The selectable markers in the cloning vector pBR322 include ampicillin and tetracycline resistance genes.
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संबंधित प्रश्न
The biological scissor is ______.
The recognition sequence of restriction enzymes is generally ______ nucleotide long.
The technique which involves addition or deletion of genes is ______.
The time taken to complete one cycle of PCR is around ______.
The vector Baculovirus is used for gene transfer in ____________.
The main reason for the presence of both a leading and a lagging strand during DNA replication is, ______
The source of taq Polymerase used in PCR is a ______.
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
The PCR technique is used for ______.
State the steps involved in the process of gene therapy for the treatment of ADA-deficiency.
