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प्रश्न
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
-
Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
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उत्तर
- The given cloning vector is called pBR322 and it is inserted into E. coli bacteria.
- In the image shown, the two restriction sites are Hind III and Bam H I.
- The selectable markers in the cloning vector pBR322 include ampicillin and tetracycline resistance genes.
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संबंधित प्रश्न
Describe two vectors less methods of gene transfer used in rDNA technology.
Which of the following is an INCORRECT match?
Identify the properties that a cloning vector must possess to be efficiently used in rDNA technology.
- Control elements like promoter, operator, ribosomal binding sites
- Restriction sites
- Marker genes
- Ori of replication
- Ability to replicate independently
Identify the process by which bacteria protect their own DNA from the attack of restriction enzymes.
Which of the following is the right combination of primers in a PCR to amplify the DNA product?
Polymerase Chain Reaction is made possible by ____________.
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
To be a cloning vector, a plasmid require ______.
Which amino acid is coded by the initiation codon?
Which one of the following enzymes is used to join DNA fragments?
