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प्रश्न
Give a reason for the following:
The diagram given below represents vector pBr322 which was modified by a research scholar Dr. Rayon, but the vector was rejected by his guide.
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उत्तर
More than one restriction site for the same RE.
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संबंधित प्रश्न
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
Explain types of enzymes used in biotechnology?
Exonuclease performs the function of ____________.
Which molecules are called molecular scissors? Why are they called so?
The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as ______.
Name the plant disease caused by Agrobacterium tumefaciens.
| Oil spill is a major environmental issue. It has been found that different strains of Pseudomonas bacteria have genes to break down the four major groups of hydrocarbons in oil. Trials are underway to use different biotechnological tools to incorporate these genes and create a genetically engineered strain of Pseudomonas - a ‘super-bug’, to break down the four major groups of hydrocarbons in oil. Such bacteria might be sprayed onto surfaces polluted with oil to clean thin films of oil. |
(a) List two advantages of using bacteria for such biotechnological studies?
(b) For amplification of the gene of interest PCR was carried out. The PCR was run with the help of polymerase which was functional only at a very low temperature. How will this impact the efficiency of the PCR? Justify.
(c) If such bacteria are sprayed on water bodies with oil spills, how will this have a positive or negative effect on the environment? Discuss.
The given figure is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly indentites its certain component(s)?
Name the enzyme responsible for the transcription of genetic code to produce mRNA.
How many sets of primers are required in each cycle of PCR?
