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प्रश्न
Give a reason for the following:
The diagram given below represents vector pBr322 which was modified by a research scholar Dr. Rayon, but the vector was rejected by his guide.
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उत्तर
More than one restriction site for the same RE.
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संबंधित प्रश्न
ECoRI is obtained from ______.
What is the role of Taq-polymerase in PCR technology?
Bt-cotton shows adverse effect on the population of which butterfly?
Explain types of enzymes used in biotechnology?
Following are the four statements regarding separation of DNA fragments using Gel electrophoresis. Identify the INCORRECT statements.
i. DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal.
ii. DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
iii. Smaller the size of DNA fragment, larger is the distance it travels through it.
iv. Pure DNA can be visualized directly by exposing to UV radiation.
EcoRl & Hind III are the examples of ______
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?
The plasmid of Agrobacterium tumefaciens that is now modified as a cloning vector is ______.
______technique is used for in vitro gene cloning or gene multiplication.
Assertion: In a bioreactor, it is not necessary to maintain sterile ambience.
Reason: Sterile conditions promote the growth of unwanted microbes in the culture medium.
