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प्रश्न
Explain the properties of a good or ideal cloning vector for rDNA technology.
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उत्तर
Following characteristic properties, a cloning vector must possess in order to be used in rDNA technology:
- A good vector should have the ability of independent replication so that as the vector replicates (through ori gene) and a large number of copies of the DNA insert will be formed.
- The vector should be able to easily introduce into host cells.
- A vector should have marker genes for antibiotic resistance.
- A vector must contain a unique cleavage site in one of the marker genes for the restriction enzyme.
- It should have at least suitable control elements like a promoter, operator, ribosomal binding sites, etc.
- The plasmids obtained naturally do not possess all the characteristics. Hence, they are constructed by inserting a gene for antibiotic resistance. eg. pBR322, pBR320, pACYC177 are the constructed plasmids. pBR322 is mostly used in rDNA technology in plants.
संबंधित प्रश्न
Identify the CORRECT sequence of steps in PCR.
______ is the soil bacterium that causes crown gall characterized by tumors in plants.
Which of the following is NOT involved in genetic engineering technique?
i. Combining genes from two organisms
ii. Gene cloning
iii. Transfer of lipid molecules
iv. Transfer of genes to new organisms
v. Repairing or replacing the defective genes by healthy genes
vi. Artificial synthesis of new gene
Restriction enzymes were discovered by ______.
The PCR technique is used for ______.
Which of the following statements are true about cloning vectors?
- Vectors are DNA molecules that carry a foreign DNA segment.
- The replicated DNA is introduced into the host cell.
- It possesses the origin of replication (Ori).
- Vectors may be plasmid, bacteriophage, Yeast artificial chromosomes (YACs), etc.
- Vectors do not require a selectable marker to identify and eliminate non-transformants.
Choose the correct answer from the options given below.
The DNA polymerase enzyme used in PCR is obtained from______.
Explain the mechanism of PCR.
Name the enzymes that are called molecular scissors.
What is the primary focus of genetic engineering?
