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Question
Explain the properties of a good or ideal cloning vector for rDNA technology.
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Solution
Following characteristic properties, a cloning vector must possess in order to be used in rDNA technology:
- A good vector should have the ability of independent replication so that as the vector replicates (through ori gene) and a large number of copies of the DNA insert will be formed.
- The vector should be able to easily introduce into host cells.
- A vector should have marker genes for antibiotic resistance.
- A vector must contain a unique cleavage site in one of the marker genes for the restriction enzyme.
- It should have at least suitable control elements like a promoter, operator, ribosomal binding sites, etc.
- The plasmids obtained naturally do not possess all the characteristics. Hence, they are constructed by inserting a gene for antibiotic resistance. eg. pBR322, pBR320, pACYC177 are the constructed plasmids. pBR322 is mostly used in rDNA technology in plants.
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