Advertisements
Advertisements
Question
Explain the properties of a good or ideal cloning vector for rDNA technology.
Advertisements
Solution
Following characteristic properties, a cloning vector must possess in order to be used in rDNA technology:
- A good vector should have the ability of independent replication so that as the vector replicates (through ori gene) and a large number of copies of the DNA insert will be formed.
- The vector should be able to easily introduce into host cells.
- A vector should have marker genes for antibiotic resistance.
- A vector must contain a unique cleavage site in one of the marker genes for the restriction enzyme.
- It should have at least suitable control elements like a promoter, operator, ribosomal binding sites, etc.
- The plasmids obtained naturally do not possess all the characteristics. Hence, they are constructed by inserting a gene for antibiotic resistance. eg. pBR322, pBR320, pACYC177 are the constructed plasmids. pBR322 is mostly used in rDNA technology in plants.
RELATED QUESTIONS
The enzyme nuclease hydrolyses ______ of polynucleotide chain of DNA.
Enlist and write in brief about the different biological tools required in rDNA technology.
Alu-I is obtained from which organism?
For which of the following purpose restriction endonuclease enzyme is used in genetic engineering?
Which of the following is an INCORRECT match?
Polymerase Chain Reaction is made possible by ____________.
Lytic cycle involves bacteriophage and ____________.
Who among the following was awarded the Nobel Prize for the development of PCR technique?
The given figure is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly indentites its certain component(s)?
Write steps involved in gene amplification.
