Advertisements
Advertisements
Question
Who among the following was awarded the Nobel Prize for the development of PCR technique?
Options
Herbert Boyer
Hargovind Khurana
Kary Mullis
Arthur Kornberg
Advertisements
Solution
Kary Mullis
APPEARS IN
RELATED QUESTIONS
What does PCR stand for? Describe the different steps of PCR
The technique which involves addition or deletion of genes is ______.
Define biotechnology? Which are the basic principles and process of biotechnology?
Identify the palindromic sequence which is recognized by the EcoRI restriction enzyme.
From the following list of cloning vectors used in rDNA technology identify the vectors that are the most commonly used.
Plasmids, bacteriophages (Ml3, lambda virus), cosmid, phagemid, BAC (bacterial artificial chromosome), YAC (yeast artificial chromosome), transposons, baculoviruses, and mammalian artificial chromosomes (MACs)
Vectors are ____________ molecules that carry a foreign gene and replicate inside the host cell.
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
Identify the properties that a cloning vector must possess to be efficiently used in rDNA technology.
- Control elements like promoter, operator, ribosomal binding sites
- Restriction sites
- Marker genes
- Ori of replication
- Ability to replicate independently
Following are the four statements regarding separation of DNA fragments using Gel electrophoresis. Identify the INCORRECT statements.
i. DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal.
ii. DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
iii. Smaller the size of DNA fragment, larger is the distance it travels through it.
iv. Pure DNA can be visualized directly by exposing to UV radiation.
Identify the CORRECT order of steps in Polymerase Chain Reaction (PCR).
In PCR technique, the organism providing thermostable DNA polymerase is a ____________.
Which of the following statements is correct with respect to cellular respiration?
Name the plant disease caused by Agrobacterium tumefaciens.
Expand the following acronym:
BAC
Which of the following enzyme catalyses the removal of nucleotides from the end of DNA molecules?
Plasmid is ______.
What is EcoRI?
Which amino acid is coded by the initiation codon?
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
-
Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
Give a reason for the following:
The diagram given below represents vector pBr322 which was modified by a research scholar Dr. Rayon, but the vector was rejected by his guide.
