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Questions
Describe two vectors less methods of gene transfer used in rDNA technology.
Describe two vectorless methods of gene transfer in rDNA technology.
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Solution
Two vectors less methods of gene transfer used in rDNA technology.
- Chemical mediated gene transfer: Use of chemicals like polyethylene glycol (PEG) and dextran sulphate induces DNA uptake by plant protoplasts.
- Electroporation: Short electric impulse of high voltage causes fine transient forces in the cell membrane through which foreign DNA enters into the cell.
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