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प्रश्न
Describe two vectors less methods of gene transfer used in rDNA technology.
Describe two vectorless methods of gene transfer in rDNA technology.
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उत्तर
Two vectors less methods of gene transfer used in rDNA technology.
- Chemical mediated gene transfer: Use of chemicals like polyethylene glycol (PEG) and dextran sulphate induces DNA uptake by plant protoplasts.
- Electroporation: Short electric impulse of high voltage causes fine transient forces in the cell membrane through which foreign DNA enters into the cell.
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संबंधित प्रश्न
Rearrange the following in the correct sequence to accomplish an important biological reaction:
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA
What would be the molar concentration of human DNA in a human cell?
Distinguish between plasmid DNA and chromosomal DNA.
What are plasmids?
How has the development of bioreactor helped in biotechnology?
What is the cell that receives a recombinant gene called?
Identify the palindromic sequence which is recognized by the EcoRI restriction enzyme.
Which one is a true statement regarding DNA polymerase used in PCR?
Which of the following enzyme catalyses the removal of nucleotides from the end of DNA molecules?
The polymerase chain reaction is a powerful technique to ______.
