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प्रश्न
Describe two vectors less methods of gene transfer used in rDNA technology.
Describe two vectorless methods of gene transfer in rDNA technology.
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उत्तर
Two vectors less methods of gene transfer used in rDNA technology.
- Chemical mediated gene transfer: Use of chemicals like polyethylene glycol (PEG) and dextran sulphate induces DNA uptake by plant protoplasts.
- Electroporation: Short electric impulse of high voltage causes fine transient forces in the cell membrane through which foreign DNA enters into the cell.
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संबंधित प्रश्न
Give the applications of the PCR technique.
Write a short note on Electrophoresis.
In vitro amplification of DNA or RNA segment is known as ___________.
The recognition sequence of restriction enzymes is generally ______ nucleotide long.
The vector Baculovirus is used for gene transfer in ____________.
The main reason for the presence of both a leading and a lagging strand during DNA replication is, ______
A hind of biotechnology involving manipulation of DNA is ______.
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
To be a cloning vector, a plasmid require ______.
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
-
Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
