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प्रश्न
Describe two vectors less methods of gene transfer used in rDNA technology.
Describe two vectorless methods of gene transfer in rDNA technology.
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उत्तर
Two vectors less methods of gene transfer used in rDNA technology.
- Chemical mediated gene transfer: Use of chemicals like polyethylene glycol (PEG) and dextran sulphate induces DNA uptake by plant protoplasts.
- Electroporation: Short electric impulse of high voltage causes fine transient forces in the cell membrane through which foreign DNA enters into the cell.
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संबंधित प्रश्न
Expand the following abbreviation: BAC
What are the restriction endonucleases? Give the rules of their nomenclature.
The enzyme restriction endonuclease ______.
Polymerase chain reaction is most useful in ______.
What is the role of Taq-polymerase in PCR technology?
Match the column I (PCR steps) and Column II (Temperature). Select the correct option.
| Column I (PCR steps) | Column II (Temperature) | ||
| i. | Polymerization | P. | 40 - 60°C |
| ii. | Denaturation | Q. | 70 - 75°C |
| iii. | Primer annealing | R. | 90 - 98°C |
| S. | 28 - 37°C |
Which molecules are called molecular scissors? Why are they called so?
The most important feature in a plasmid to serve as a vector in gene cloning experiment is ______.
Name a recombinant vaccine that is currently being used in vaccination programme.
Expand the following acronym:
BAC
