Advertisements
Advertisements
प्रश्न
Assertion: In a bioreactor, it is not necessary to maintain sterile ambience.
Reason: Sterile conditions promote the growth of unwanted microbes in the culture medium.
पर्याय
Both Assertion and Reason are true, and Reason is the correct explanation for Assertion.
Both Assertion and Reason are true, but Reason is not the correct explanation for Assertion.
Assertion is true and Reason is false.
Both Assertion and Reason are false.
Advertisements
उत्तर
Both Assertion and Reason are false.
Explanation:
Maintaining sterile conditions in a bioreactor is crucial to prevent unwanted bacteria from growing in the culture medium during product production.
APPEARS IN
संबंधित प्रश्न
What are the restriction endonucleases? Give the rules of their nomenclature.
What is Palindromic sequence?
For which of the following reason Agrobacterium tumefaciens is most widely used for gene transfer in plants?
Identify the characterization techniques that are based on molecular weights of compounds.
Which of the following device is required for PCR?
DNA fingerprinting refers to ______.
Annealing step of PCR operates at ______ °C.
| Oil spill is a major environmental issue. It has been found that different strains of Pseudomonas bacteria have genes to break down the four major groups of hydrocarbons in oil. Trials are underway to use different biotechnological tools to incorporate these genes and create a genetically engineered strain of Pseudomonas - a ‘super-bug’, to break down the four major groups of hydrocarbons in oil. Such bacteria might be sprayed onto surfaces polluted with oil to clean thin films of oil. |
(a) List two advantages of using bacteria for such biotechnological studies?
(b) For amplification of the gene of interest PCR was carried out. The PCR was run with the help of polymerase which was functional only at a very low temperature. How will this impact the efficiency of the PCR? Justify.
(c) If such bacteria are sprayed on water bodies with oil spills, how will this have a positive or negative effect on the environment? Discuss.
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
Suneeta is planning an experiment to clone a gene in a vector. So, she has to choose a good cloning vector.
Which one of the vectors shown below should she choose? Justify your answer by giving two reasons.
 |
 |
 |
| Vector A | Vector B | Vector C |
