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प्रश्न
Which of the following is not required in the preparation of a recombinant DNA molecule?
पर्याय
Restriction endonuclease
DNA ligase
DNA fragments
E.coli
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उत्तर
E.coli
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संबंधित प्रश्न
What does PCR stand for? Describe the different steps of PCR
The biological scissor is ______.
Give the applications of the PCR technique.
Write a short note on Electrophoresis.
Expand the following abbreviation: BAC
The bacterium which causes a plant disease called crown gall is _______________.
Define vector? write any two examples,
From the following list of cloning vectors used in rDNA technology identify the vectors that are the most commonly used.
Plasmids, bacteriophages (Ml3, lambda virus), cosmid, phagemid, BAC (bacterial artificial chromosome), YAC (yeast artificial chromosome), transposons, baculoviruses, and mammalian artificial chromosomes (MACs)
For which of the following reason Agrobacterium tumefaciens is most widely used for gene transfer in plants?
______ is the soil bacterium that causes crown gall characterized by tumors in plants.
Study the following statements regarding separation of DNA using agarose gel electrophoresis.
- Large fragments of DNA molecules move faster than small DNA fragments and thus separate faster.
- DNA being negatively charged migrates to cathode.
Which of the following is NOT involved in genetic engineering technique?
i. Combining genes from two organisms
ii. Gene cloning
iii. Transfer of lipid molecules
iv. Transfer of genes to new organisms
v. Repairing or replacing the defective genes by healthy genes
vi. Artificial synthesis of new gene
____________ is the manipulation of genetic material towards a desired end, in a directed and predetermined way, using in vitro process.
Polymerase Chain Reaction is made possible by ____________.
The most important feature in a plasmid to serve as a vector in gene cloning experiment is ______.
Expand the following acronym:
BAC
The given figure is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly indentites its certain component(s)?

Write a short note on vectors.
Suneeta is planning an experiment to clone a gene in a vector. So, she has to choose a good cloning vector.
Which one of the vectors shown below should she choose? Justify your answer by giving two reasons.
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| Vector A | Vector B | Vector C |



