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प्रश्न
Which of the following is not required in the preparation of a recombinant DNA molecule?
पर्याय
Restriction endonuclease
DNA ligase
DNA fragments
E.coli
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उत्तर
E.coli
APPEARS IN
संबंधित प्रश्न
Explain the work carried out by Cohen and Boyer that contributed immensely in biotechnology.
Distinguish between plasmid DNA and chromosomal DNA.
The enzyme nuclease hydrolyses ______ of polynucleotide chain of DNA.
Explain the properties of a good or ideal cloning vector for rDNA technology.
Give the steps in PCR or polymerase chain reaction with suitable diagrams.
Bt-cotton shows adverse effect on the population of which butterfly?
Define vector? write any two examples,
What is Recognition sequence? Explain in brief.
From the following which one is NOT a feature of the plasmids?
Identify the characterization techniques that are based on molecular weights of compounds.
The following identifies the major features of technology that differentiate modem biotechnology from classical biotechnology.
i. Capability of science to change the genetic material for getting new specific products through rDNA technology, polymerase chain reaction (PCR), microarrays, cell culture and fusion, and bioprocessing.
ii. Ownership of technology and its sociopolitical impact.
iii. Modem biotechnology makes use of only fermentation technology.
iv. Classical biotechnology relies only on principles of plant and animal tissue culture.
Which of the following is the right combination of primers in a PCR to amplify the DNA product?
Lytic cycle involves bacteriophage and ____________.
EcoRl & Hind III are the examples of ______
Which of the following statements is correct with respect to cellular respiration?
Name the plant disease caused by Agrobacterium tumefaciens.
Which of the following is the correct recognition sequence of restriction enzyme Eco RI?
Which of the following enzyme catalyses the removal of nucleotides from the end of DNA molecules?
pBR322, used very frequently in cloning experiments as a vector in E. coli is ______.
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
