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प्रश्न
Which of the following is not required in the preparation of a recombinant DNA molecule?
पर्याय
Restriction endonuclease
DNA ligase
DNA fragments
E.coli
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उत्तर
E.coli
APPEARS IN
संबंधित प्रश्न
Name and explain the technique used for separating DNA fragments and making them available for biotechnology experiments.
Explain what are the desirable characteristics of an ideal cloning vector used in rDNA technology.
Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
The enzyme nuclease hydrolyses ______ of polynucleotide chain of DNA.
The recognition sequence of restriction enzymes is generally ______ nucleotide long.
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
DNA polymerase enzyme used in PCR is isolated from ____________ bacteria.
Which of the following DNA sequence is recognised and cut by EcoR I?
EcoRl & Hind III are the examples of ______
The rDNA technology has provided a method to control the nematode parasite Meloidogyne incognita. Explain the principle involved in this technique.
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
Identify the desirable characteristics for a plasmid used in rDNA technology from the following.
- Ability to multiply and express outside the host in a bioreactor.
- A highly active promoter.
- A site at which replication can be initiated.
- One or more identifiable marker genes.
- One or more unique restriction sites.
Which of these are most widely used in genetic engineering?
Describe three steps involved in mechanism of PCR.
To be a cloning vector, a plasmid require ______.
______technique is used for in vitro gene cloning or gene multiplication.
What is the importance of PCR.
State the steps involved in the process of gene therapy for the treatment of ADA-deficiency.
Suneeta is planning an experiment to clone a gene in a vector. So, she has to choose a good cloning vector.
Which one of the vectors shown below should she choose? Justify your answer by giving two reasons.
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| Vector A | Vector B | Vector C |
