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प्रश्न
Which of the following is not required in the preparation of a recombinant DNA molecule?
पर्याय
Restriction endonuclease
DNA ligase
DNA fragments
E.coli
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उत्तर
E.coli
APPEARS IN
संबंधित प्रश्न
Retroviruses have no DNA. However, the DNA of the infected host cell does possess viral DNA. How is it possible?
Name and explain the technique used for separating DNA fragments and making them available for biotechnology experiments.
Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
ECoRI is obtained from ______.
Ti plasmid being used for introducing genes in plants obtained from ______.
Polymerase chain reaction is most useful in ______.
Explain types of enzymes used in biotechnology?
DNA polymerase enzyme used in PCR is isolated from ____________ bacteria.
Match the Column I (Restriction enzyme) with Column II (Recognition sequence) and select the correct option.
| column I (Restriction Enzyme) | column II (Recognition sequence) | ||
| i. | Alu | P. | 5'---G- ↓-G-A-T-C-C---3' 3'---C-C-T-A-G- -G---5 |
| ii. | BamHI | Q. | 5'--- A-G- ↓ -C-T---3' 3' ---T-C- G-A--- 5' |
| iii. | Eco RI | R. | 5'--G-T-C- ↓ -G-A-C--3' I 3'--C-A-G-t-C-T-G--5' |
| iv. | HindII | S. | 5'---G- ↓ -A-A-T-T-C---3' 3'---C-T-T-A-A- -G---5' |
In PCR technique, the organism providing thermostable DNA polymerase is a ____________.
The rDNA technology has provided a method to control the nematode parasite Meloidogyne incognita. Explain the principle involved in this technique.
Name a recombinant vaccine that is currently being used in vaccination programme.
Name the plant disease caused by Agrobacterium tumefaciens.
Restriction enzymes were discovered by ______.
The PCR technique is used for ______.
Which vector is mostly used in rDNA technology in plants?
Study the following lists and choose the correct match.
| List I | List II |
| A. Vector | (i) Resistant to cotton bollworms |
| B. Downstream processing | (ii) Mobile genetic elements |
| C. Cry II AB | (iii) Controls corn borers |
| D. Transposons | (iv) Ti plasmid |
| (v) Purifying protein in biopharmaceuticals |
Expand YAC used in the field of Biotechnology.
Which one of the following enzymes is used to join DNA fragments?
Suneeta is planning an experiment to clone a gene in a vector. So, she has to choose a good cloning vector.
Which one of the vectors shown below should she choose? Justify your answer by giving two reasons.
 |
 |
 |
| Vector A | Vector B | Vector C |
