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प्रश्न
What is the role of Taq-polymerase in PCR technology?
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उत्तर
Taq-polymerase helps in polymerization of DNA molecules in PCR technology.
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संबंधित प्रश्न
Name and explain the technique used for separating DNA fragments and making them available for biotechnology experiments.
Why are bio-fertilizers preferred over chemical fertilizers?
In vitro amplification of DNA or RNA segment is known as ___________.
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
Nucleic acid segment tagged with a radioactive molecule is called ______.
What is the significance of adding proteases at the time of isolation of genetic material (DNA)?
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
Write steps involved in gene amplification.
Name the enzyme responsible for the transcription of genetic code to produce mRNA.
Suneeta is planning an experiment to clone a gene in a vector. So, she has to choose a good cloning vector.
Which one of the vectors shown below should she choose? Justify your answer by giving two reasons.
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 |
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| Vector A | Vector B | Vector C |
