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प्रश्न
Name two restriction enzymes used in PCR.
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उत्तर
- EcoRI
- Hind Ill
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संबंधित प्रश्न
What would be the molar concentration of human DNA in a human cell?
Explain the properties of a good or ideal cloning vector for rDNA technology.
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Define vector? write any two examples,
DNA polymerase enzyme used in PCR is isolated from ____________ bacteria.
Consider there is only one double stranded DNA molecule initially along with all other requirements of PCR. If a PCR machines performs 10 cycles of reaction then bow many double stranded DNA molecules would be present at the end?
The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as ______.
Annealing step of PCR operates at ______ °C.
Which of the following statements are true about cloning vectors?
- Vectors are DNA molecules that carry a foreign DNA segment.
- The replicated DNA is introduced into the host cell.
- It possesses the origin of replication (Ori).
- Vectors may be plasmid, bacteriophage, Yeast artificial chromosomes (YACs), etc.
- Vectors do not require a selectable marker to identify and eliminate non-transformants.
Choose the correct answer from the options given below.
The polymerase chain reaction is a powerful technique to ______.
