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प्रश्न
Name two restriction enzymes used in PCR.
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उत्तर
- EcoRI
- Hind Ill
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संबंधित प्रश्न
The enzyme restriction endonuclease ______.
What is plasmid?
What is the role of Taq-polymerase in PCR technology?
What is meant by gene cloning?
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
pBR322, used very frequently in cloning experiments as a vector in E. coli is ______.
Name the enzyme responsible for the transcription of genetic code to produce mRNA.
What is the importance of PCR.
How many sets of primers are required in each cycle of PCR?
