Advertisements
Advertisements
प्रश्न
Name two restriction enzymes used in PCR.
Advertisements
उत्तर
- EcoRI
- Hind Ill
APPEARS IN
संबंधित प्रश्न
Rearrange the following in the correct sequence to accomplish an important biological reaction:
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA
A PCR machine can raise the temperature up to 100°C but after that, it is not able to lower the temperature below 70°C automatically. Which step of PCR will be hampered first in this faulty machine? Explain why?
Enlist different types of restriction enzymes commonly used in rDNA technology? Write about their role.
Identify the CORRECT sequence of steps in PCR.
From which of the following organisms the enzyme Taq polymerase used in PCR technique is obtained?
Which one is a true statement regarding DNA polymerase used in PCR?
The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as ______.
Annealing step of PCR operates at ______ °C.
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
How many sets of primers are required in each cycle of PCR?
