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प्रश्न
Name two restriction enzymes used in PCR.
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उत्तर
- EcoRI
- Hind Ill
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संबंधित प्रश्न
The bacterium which causes a plant disease called crown gall is _______________.
The technique which involves addition or deletion of genes is ______.
The enzyme restriction endonuclease ______.
Define biotechnology? Which are the basic principles and process of biotechnology?
Identify the process by which bacteria protect their own DNA from the attack of restriction enzymes.
Following are the four statements regarding separation of DNA fragments using Gel electrophoresis. Identify the INCORRECT statements.
i. DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal.
ii. DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
iii. Smaller the size of DNA fragment, larger is the distance it travels through it.
iv. Pure DNA can be visualized directly by exposing to UV radiation.
A technique used to make numerous copies of a specific DNA segment quickly and accurately is called ______
The source of taq Polymerase used in PCR is a ______.
Which of the following statements are true about cloning vectors?
- Vectors are DNA molecules that carry a foreign DNA segment.
- The replicated DNA is introduced into the host cell.
- It possesses the origin of replication (Ori).
- Vectors may be plasmid, bacteriophage, Yeast artificial chromosomes (YACs), etc.
- Vectors do not require a selectable marker to identify and eliminate non-transformants.
Choose the correct answer from the options given below.
How gene amplification is done?
