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प्रश्न
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?
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उत्तर
When a DNA molecule is created by ligating a gene to a plasmid vector, It becomes a circular DNA which is ready to replicate in host organism. After this stage, addition of exonuclease is not going to affect the process because the DNA does not have a free end and hence enzyme exonuclease will not get a substrate to show its action. So, in this experiment; bacterial transformation is not going to be disturbed.
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संबंधित प्रश्न
Distinguish between RNA and DNA.
Define vector? write any two examples,
What is PCR? Explain different steps involved in it.
From the following list of cloning vectors used in rDNA technology identify the vectors that are the most commonly used.
Plasmids, bacteriophages (Ml3, lambda virus), cosmid, phagemid, BAC (bacterial artificial chromosome), YAC (yeast artificial chromosome), transposons, baculoviruses, and mammalian artificial chromosomes (MACs)
Identify the process by which bacteria protect their own DNA from the attack of restriction enzymes.
Which of the following is the right combination of primers in a PCR to amplify the DNA product?
Match the column I (PCR steps) and Column II (Temperature). Select the correct option.
| Column I (PCR steps) | Column II (Temperature) | ||
| i. | Polymerization | P. | 40 - 60°C |
| ii. | Denaturation | Q. | 70 - 75°C |
| iii. | Primer annealing | R. | 90 - 98°C |
| S. | 28 - 37°C |
The vector Baculovirus is used for gene transfer in ____________.
DNA fingerprinting refers to ______.
The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as ______.
Which of the following is not required in the preparation of a recombinant DNA molecule?
Which vector is mostly used in rDNA technology in plants?
What is polymerase chain reaction (PCR)?
The plasmid of Agrobacterium tumefaciens that is now modified as a cloning vector is ______.
What are the different types of electrophoresis?
Name the enzyme responsible for the transcription of genetic code to produce mRNA.
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
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Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
David is a molecular biologist. He uses a technique to know the amino acids occupy the first and the last positions in the Chain - A and Chain - B of insulin. Name the scientist whose contribution made it possible for David to use this technique.
The restriction enzymes used as molecular scissors are type of ______.
