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प्रश्न
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?
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उत्तर
When a DNA molecule is created by ligating a gene to a plasmid vector, It becomes a circular DNA which is ready to replicate in host organism. After this stage, addition of exonuclease is not going to affect the process because the DNA does not have a free end and hence enzyme exonuclease will not get a substrate to show its action. So, in this experiment; bacterial transformation is not going to be disturbed.
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संबंधित प्रश्न
Retroviruses have no DNA. However, the DNA of the infected host cell does possess viral DNA. How is it possible?
What are plasmids?
Give the applications of the PCR technique.
What are the restriction endonucleases? Give the rules of their nomenclature.
The technique which involves addition or deletion of genes is ______.
The enzyme restriction endonuclease ______.
What is Palindromic sequence?
For which of the following purpose restriction endonuclease enzyme is used in genetic engineering?
Which of the following is an INCORRECT match?
Identify the properties that a cloning vector must possess to be efficiently used in rDNA technology.
- Control elements like promoter, operator, ribosomal binding sites
- Restriction sites
- Marker genes
- Ori of replication
- Ability to replicate independently
Consider there is only one double stranded DNA molecule initially along with all other requirements of PCR. If a PCR machines performs 10 cycles of reaction then bow many double stranded DNA molecules would be present at the end?
From which of the following organisms the enzyme Taq polymerase used in PCR technique is obtained?
Which of the following is the right combination of primers in a PCR to amplify the DNA product?
The gene transfer between unrelated plants is brought about by ____________.
Exonuclease performs the function of ____________.
Which of the following viruses has the capacity to produce lysozymes?
What is true for plasmid?
What are the different types of electrophoresis?
Give a reason for the following:
The diagram given below represents vector pBr322 which was modified by a research scholar Dr. Rayon, but the vector was rejected by his guide.
