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प्रश्न
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?
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उत्तर
When a DNA molecule is created by ligating a gene to a plasmid vector, It becomes a circular DNA which is ready to replicate in host organism. After this stage, addition of exonuclease is not going to affect the process because the DNA does not have a free end and hence enzyme exonuclease will not get a substrate to show its action. So, in this experiment; bacterial transformation is not going to be disturbed.
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संबंधित प्रश्न
What are plasmids?
Name the most commonly used bioreactor and describe its working.
Give one significant difference between:
Electroporation and Gene Gun.
Explain what are the desirable characteristics of an ideal cloning vector used in rDNA technology.
Which of the following is TRUE regarding EcoR I?
Which of the following is an INCORRECT match?
The following identifies the major features of technology that differentiate modem biotechnology from classical biotechnology.
i. Capability of science to change the genetic material for getting new specific products through rDNA technology, polymerase chain reaction (PCR), microarrays, cell culture and fusion, and bioprocessing.
ii. Ownership of technology and its sociopolitical impact.
iii. Modem biotechnology makes use of only fermentation technology.
iv. Classical biotechnology relies only on principles of plant and animal tissue culture.
______ is a Type I restriction enzyme.
The gene transfer between unrelated plants is brought about by ____________.
EcoRl & Hind III are the examples of ______
Name and describe the technique that helps in separation of DNA fragments.
Nucleic acid segment tagged with a radioactive molecule is called ______.
DNA fingerprinting refers to ______.
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
Which of the following is the correct recognition sequence of restriction enzyme Eco RI?
Describe three steps involved in mechanism of PCR.
To be a cloning vector, a plasmid require ______.
How gene amplification is done?
Name the enzymes that are called molecular scissors.
