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प्रश्न
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?
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उत्तर
When a DNA molecule is created by ligating a gene to a plasmid vector, It becomes a circular DNA which is ready to replicate in host organism. After this stage, addition of exonuclease is not going to affect the process because the DNA does not have a free end and hence enzyme exonuclease will not get a substrate to show its action. So, in this experiment; bacterial transformation is not going to be disturbed.
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संबंधित प्रश्न
Name the most commonly used bioreactor and describe its working.
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
Explain what are the desirable characteristics of an ideal cloning vector used in rDNA technology.
What is the cell that receives a recombinant gene called?
What is gene cloning? Explain different tools used for it.
What is Recognition sequence? Explain in brief.
From the following list of cloning vectors used in rDNA technology identify the vectors that are the most commonly used.
Plasmids, bacteriophages (Ml3, lambda virus), cosmid, phagemid, BAC (bacterial artificial chromosome), YAC (yeast artificial chromosome), transposons, baculoviruses, and mammalian artificial chromosomes (MACs)
Which of the following is an INCORRECT match?
Which of the following is NOT involved in genetic engineering technique?
i. Combining genes from two organisms
ii. Gene cloning
iii. Transfer of lipid molecules
iv. Transfer of genes to new organisms
v. Repairing or replacing the defective genes by healthy genes
vi. Artificial synthesis of new gene
In PCR technique, the organism providing thermostable DNA polymerase is a ____________.
The vector Baculovirus is used for gene transfer in ____________.
EcoRl & Hind III are the examples of ______
Which molecules are called molecular scissors? Why are they called so?
The rDNA technology has provided a method to control the nematode parasite Meloidogyne incognita. Explain the principle involved in this technique.
Which of the following is not required in the preparation of a recombinant DNA molecule?
Name a recombinant vaccine that is currently being used in vaccination programme.
Write a short note on vectors.
What is the importance of PCR.
The manipulation of genetic material towards a desired end using "in-vitro" process is called______.
When the phage DNA enters the host bacterium, the host cell protects itself from the viral DNA attacks with the help of ______ enzyme.
