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प्रश्न
Give one significant difference between:
Electroporation and Gene Gun.
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उत्तर
Electroporation: A method for increasing DNA uptake by protoplast through prior exposure to high voltage which results in the temporary formation of small pore in cell membranes.
Gene Gun: It is the vectorless transfer of gene where tungsten or gold particles coating with desired genes are bombarded into the cells with great force.
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संबंधित प्रश्न
What would be the molar concentration of human DNA in a human cell?
Distinguish between plasmid DNA and chromosomal DNA.
Give the applications of the PCR technique.
Define vector? write any two examples,
Match the Column I (Restriction enzyme) with Column II (Recognition sequence) and select the correct option.
| column I (Restriction Enzyme) | column II (Recognition sequence) | ||
| i. | Alu | P. | 5'---G- ↓-G-A-T-C-C---3' 3'---C-C-T-A-G- -G---5 |
| ii. | BamHI | Q. | 5'--- A-G- ↓ -C-T---3' 3' ---T-C- G-A--- 5' |
| iii. | Eco RI | R. | 5'--G-T-C- ↓ -G-A-C--3' I 3'--C-A-G-t-C-T-G--5' |
| iv. | HindII | S. | 5'---G- ↓ -A-A-T-T-C---3' 3'---C-T-T-A-A- -G---5' |
In which of the following techniques, charged molecules (DNA, RNA and Proteins) are separated by applying the electric field?
The gene transfer between unrelated plants is brought about by ____________.
Name two restriction enzymes used in PCR.
pBR322, used very frequently in cloning experiments as a vector in E. coli is ______.
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
