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प्रश्न
Give one significant difference between:
Electroporation and Gene Gun.
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उत्तर
Electroporation: A method for increasing DNA uptake by protoplast through prior exposure to high voltage which results in the temporary formation of small pore in cell membranes.
Gene Gun: It is the vectorless transfer of gene where tungsten or gold particles coating with desired genes are bombarded into the cells with great force.
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संबंधित प्रश्न
Name and explain the technique used for separating DNA fragments and making them available for biotechnology experiments.
In vitro amplification of DNA or RNA segment is known as ___________.
Identify the palindromic sequence which is recognized by the EcoRI restriction enzyme.
The time taken to complete one cycle of PCR is around ______.
______ is a Type I restriction enzyme.
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?
Name two restriction enzymes used in PCR.
What are the different types of electrophoresis?
Which amino acid is coded by the initiation codon?
How many sets of primers are required in each cycle of PCR?
