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प्रश्न
Give one significant difference between:
Electroporation and Gene Gun.
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उत्तर
Electroporation: A method for increasing DNA uptake by protoplast through prior exposure to high voltage which results in the temporary formation of small pore in cell membranes.
Gene Gun: It is the vectorless transfer of gene where tungsten or gold particles coating with desired genes are bombarded into the cells with great force.
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संबंधित प्रश्न
Rearrange the following in the correct sequence to accomplish an important biological reaction:
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA
How has the development of bioreactor helped in biotechnology?
Give the steps in PCR or polymerase chain reaction with suitable diagrams.
What is plasmid?
Match the List I with List II and List ill. Select the correct option.
| List I | List II | List ill | |||
| i. | Hind III | a. | Agarose ge | 1. | Six base pairs |
| ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marke |
| iii. | T-DNA | c. | Ampicillin | 3. | Elution |
| iv. | DNA | d. | Recognition sequenc | 4. | Transgenic plant |
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
DNA polymerase enzyme used in PCR is isolated from ____________ bacteria.
In PCR technique, the organism providing thermostable DNA polymerase is a ____________.
The main reason for the presence of both a leading and a lagging strand during DNA replication is, ______
The rDNA technology has provided a method to control the nematode parasite Meloidogyne incognita. Explain the principle involved in this technique.
