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प्रश्न
Give one significant difference between:
Electroporation and Gene Gun.
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उत्तर
Electroporation: A method for increasing DNA uptake by protoplast through prior exposure to high voltage which results in the temporary formation of small pore in cell membranes.
Gene Gun: It is the vectorless transfer of gene where tungsten or gold particles coating with desired genes are bombarded into the cells with great force.
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संबंधित प्रश्न
Name the most commonly used bioreactor and describe its working.
The technique which involves addition or deletion of genes is ______.
Ti plasmid being used for introducing genes in plants obtained from ______.
From the following list of cloning vectors used in rDNA technology identify the vectors that are the most commonly used.
Plasmids, bacteriophages (Ml3, lambda virus), cosmid, phagemid, BAC (bacterial artificial chromosome), YAC (yeast artificial chromosome), transposons, baculoviruses, and mammalian artificial chromosomes (MACs)
The time taken to complete one cycle of PCR is around ______.
______ is a Type I restriction enzyme.
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?
To be a cloning vector, a plasmid require ______.
David is a molecular biologist. He uses a technique to know the amino acids occupy the first and the last positions in the Chain - A and Chain - B of insulin. Name the scientist whose contribution made it possible for David to use this technique.
When the phage DNA enters the host bacterium, the host cell protects itself from the viral DNA attacks with the help of ______ enzyme.
