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प्रश्न
What is gene cloning? Explain different tools used for it.
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उत्तर
- Gene cloning is a process which involves the isolation, transfer of a specific sequence of DNA through a vector and amplification in a host organism.
- There are three types of biological tools used in rDNA technology viz. enzymes, cloning vectors (vehicle DNA) and competent host (cloning organisms) for transformation with recombinant DNA.
संबंधित प्रश्न
Give an account of the Blue-White Method of selection of recombinants.
Explain the properties of a good or ideal cloning vector for rDNA technology.
ECoRI is obtained from ______.
Bt-cotton shows adverse effect on the population of which butterfly?
From the following list of cloning vectors used in rDNA technology identify the vectors that are the most commonly used.
Plasmids, bacteriophages (Ml3, lambda virus), cosmid, phagemid, BAC (bacterial artificial chromosome), YAC (yeast artificial chromosome), transposons, baculoviruses, and mammalian artificial chromosomes (MACs)
Polymerase Chain Reaction is made possible by ____________.
pBR322, used very frequently in cloning experiments as a vector in E. coli is ______.
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
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Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
When the phage DNA enters the host bacterium, the host cell protects itself from the viral DNA attacks with the help of ______ enzyme.
According to the European Federation of Biotechnology (EFB), biotechnology is defined as:
