Advertisements
Advertisements
प्रश्न
What is gene cloning? Explain different tools used for it.
Advertisements
उत्तर
- Gene cloning is a process which involves the isolation, transfer of a specific sequence of DNA through a vector and amplification in a host organism.
- There are three types of biological tools used in rDNA technology viz. enzymes, cloning vectors (vehicle DNA) and competent host (cloning organisms) for transformation with recombinant DNA.
संबंधित प्रश्न
Explain the properties of a good or ideal cloning vector for rDNA technology.
Define vector? write any two examples,
______ is the soil bacterium that causes crown gall characterized by tumors in plants.
Identify the CORRECT order of steps in Polymerase Chain Reaction (PCR).
The gene transfer between unrelated plants is brought about by ____________.
The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as ______.
pBR322, used very frequently in cloning experiments as a vector in E. coli is ______.
In vitro amplification of DNA segments is called______.
When the phage DNA enters the host bacterium, the host cell protects itself from the viral DNA attacks with the help of ______ enzyme.
The restriction enzymes used as molecular scissors are type of ______.
