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प्रश्न
What is gene cloning? Explain different tools used for it.
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उत्तर
- Gene cloning is a process which involves the isolation, transfer of a specific sequence of DNA through a vector and amplification in a host organism.
- There are three types of biological tools used in rDNA technology viz. enzymes, cloning vectors (vehicle DNA) and competent host (cloning organisms) for transformation with recombinant DNA.
संबंधित प्रश्न
Describe two vectors less methods of gene transfer used in rDNA technology.
What is the cell that receives a recombinant gene called?
Explain the properties of a good or ideal cloning vector for rDNA technology.
A PCR machine can raise the temperature up to 100°C but after that, it is not able to lower the temperature below 70°C automatically. Which step of PCR will be hampered first in this faulty machine? Explain why?
Identify the palindromic sequence which is recognized by the EcoRI restriction enzyme.
For which of the following purpose restriction endonuclease enzyme is used in genetic engineering?
Describe three steps involved in mechanism of PCR.
When the phage DNA enters the host bacterium, the host cell protects itself from the viral DNA attacks with the help of ______ enzyme.
Who accomplished the creation of the first artificial recombinant DNA molecule, and what was linked together?
Which of the following best describes the relationship between genetic engineering and recombinant DNA?
