Advertisements
Advertisements
Question
What is gene cloning? Explain different tools used for it.
Advertisements
Solution
- Gene cloning is a process which involves the isolation, transfer of a specific sequence of DNA through a vector and amplification in a host organism.
- There are three types of biological tools used in rDNA technology viz. enzymes, cloning vectors (vehicle DNA) and competent host (cloning organisms) for transformation with recombinant DNA.
RELATED QUESTIONS
Describe two vectors less methods of gene transfer used in rDNA technology.
Enlist different types of restriction enzymes commonly used in rDNA technology? Write about their role.
Define vector? write any two examples,
A technique used to make numerous copies of a specific DNA segment quickly and accurately is called ______
Which molecules are called molecular scissors? Why are they called so?
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
______technique is used for in vitro gene cloning or gene multiplication.
What are the different types of electrophoresis?
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
-
Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
Give a reason for the following:
The diagram given below represents vector pBr322 which was modified by a research scholar Dr. Rayon, but the vector was rejected by his guide.
