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प्रश्न
What is gene cloning? Explain different tools used for it.
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उत्तर
- Gene cloning is a process which involves the isolation, transfer of a specific sequence of DNA through a vector and amplification in a host organism.
- There are three types of biological tools used in rDNA technology viz. enzymes, cloning vectors (vehicle DNA) and competent host (cloning organisms) for transformation with recombinant DNA.
संबंधित प्रश्न
What would be the molar concentration of human DNA in a human cell?
Distinguish between RNA and DNA.
The enzyme nuclease hydrolyses ______ of polynucleotide chain of DNA.
What is Recognition sequence? Explain in brief.
A technique used to make numerous copies of a specific DNA segment quickly and accurately is called ______
Who among the following was awarded the Nobel Prize for the development of PCR technique?
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
Which vector is mostly used in rDNA technology in plants?
Describe three steps involved in mechanism of PCR.
Name the source of thermostable DNA polymerase.
