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प्रश्न
What is gene cloning? Explain different tools used for it.
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उत्तर
- Gene cloning is a process which involves the isolation, transfer of a specific sequence of DNA through a vector and amplification in a host organism.
- There are three types of biological tools used in rDNA technology viz. enzymes, cloning vectors (vehicle DNA) and competent host (cloning organisms) for transformation with recombinant DNA.
संबंधित प्रश्न
Distinguish between RNA and DNA.
Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
In vitro amplification of DNA or RNA segment is known as ___________.
The rDNA technology has provided a method to control the nematode parasite Meloidogyne incognita. Explain the principle involved in this technique.
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
Identify the desirable characteristics for a plasmid used in rDNA technology from the following.
- Ability to multiply and express outside the host in a bioreactor.
- A highly active promoter.
- A site at which replication can be initiated.
- One or more identifiable marker genes.
- One or more unique restriction sites.
The polymerase chain reaction is a powerful technique to ______.
How gene amplification is done?
In vitro amplification of DNA segments is called______.
How many sets of primers are required in each cycle of PCR?
