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प्रश्न
What is gene cloning? Explain different tools used for it.
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उत्तर
- Gene cloning is a process which involves the isolation, transfer of a specific sequence of DNA through a vector and amplification in a host organism.
- There are three types of biological tools used in rDNA technology viz. enzymes, cloning vectors (vehicle DNA) and competent host (cloning organisms) for transformation with recombinant DNA.
संबंधित प्रश्न
What is the cell that receives a recombinant gene called?
DNA polymerase enzyme used in PCR is isolated from ____________ bacteria.
Following are the four statements regarding separation of DNA fragments using Gel electrophoresis. Identify the INCORRECT statements.
i. DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal.
ii. DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
iii. Smaller the size of DNA fragment, larger is the distance it travels through it.
iv. Pure DNA can be visualized directly by exposing to UV radiation.
Polymerase Chain Reaction is made possible by ____________.
The source of taq Polymerase used in PCR is a ______.
The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as ______.
Name a recombinant vaccine that is currently being used in vaccination programme.
Which of these are most widely used in genetic engineering?
pBR322, used very frequently in cloning experiments as a vector in E. coli is ______.
What is EcoRI?
