Advertisements
Advertisements
प्रश्न
What is gene cloning? Explain different tools used for it.
Advertisements
उत्तर
- Gene cloning is a process which involves the isolation, transfer of a specific sequence of DNA through a vector and amplification in a host organism.
- There are three types of biological tools used in rDNA technology viz. enzymes, cloning vectors (vehicle DNA) and competent host (cloning organisms) for transformation with recombinant DNA.
संबंधित प्रश्न
Give an account of the Blue-White Method of selection of recombinants.
The recognition sequence of restriction enzymes is generally ______ nucleotide long.
Bt-cotton shows adverse effect on the population of which butterfly?
From the following list of cloning vectors used in rDNA technology identify the vectors that are the most commonly used.
Plasmids, bacteriophages (Ml3, lambda virus), cosmid, phagemid, BAC (bacterial artificial chromosome), YAC (yeast artificial chromosome), transposons, baculoviruses, and mammalian artificial chromosomes (MACs)
In which of the following techniques, charged molecules (DNA, RNA and Proteins) are separated by applying the electric field?
Which of the following is the right combination of primers in a PCR to amplify the DNA product?
____________ is the manipulation of genetic material towards a desired end, in a directed and predetermined way, using in vitro process.
Nucleic acid segment tagged with a radioactive molecule is called ______.
Which of these are most widely used in genetic engineering?
The DNA polymerase enzyme used in PCR is obtained from______.
