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प्रश्न
Expand the following abbreviation: BAC
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उत्तर
Bacterial Artificial Chromosome
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संबंधित प्रश्न
Give an account of the Blue-White Method of selection of recombinants.
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
Enlist different types of restriction enzymes commonly used in rDNA technology? Write about their role.
Match the column I (PCR steps) and Column II (Temperature). Select the correct option.
| Column I (PCR steps) | Column II (Temperature) | ||
| i. | Polymerization | P. | 40 - 60°C |
| ii. | Denaturation | Q. | 70 - 75°C |
| iii. | Primer annealing | R. | 90 - 98°C |
| S. | 28 - 37°C |
Who among the following was awarded the Nobel Prize for the development of PCR technique?
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
Name two restriction enzymes used in PCR.
Describe three steps involved in mechanism of PCR.
Explain the mechanism of PCR.
Assertion: In a bioreactor, it is not necessary to maintain sterile ambience.
Reason: Sterile conditions promote the growth of unwanted microbes in the culture medium.
