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प्रश्न
Name the source of thermostable DNA polymerase.
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उत्तर
A bacterium Thermus aquaticus.
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संबंधित प्रश्न
In vitro amplification of DNA or RNA segment is known as ___________.
Explain the properties of a good or ideal cloning vector for rDNA technology.
____________ is the manipulation of genetic material towards a desired end, in a directed and predetermined way, using in vitro process.
A technique used to make numerous copies of a specific DNA segment quickly and accurately is called ______
The main reason for the presence of both a leading and a lagging strand during DNA replication is, ______
The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as ______.
Identify the desirable characteristics for a plasmid used in rDNA technology from the following.
- Ability to multiply and express outside the host in a bioreactor.
- A highly active promoter.
- A site at which replication can be initiated.
- One or more identifiable marker genes.
- One or more unique restriction sites.
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
How gene amplification is done?
Write steps involved in gene amplification.
