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प्रश्न
Explain types of enzymes used in biotechnology?
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उत्तर
| No | Type of enzyme | Examples | Functions |
| i. | Lysing enzyme | Lysozyme | Digesting bacterial cell walls |
| ii. | Cleaving enzymes (Molecular scissors) | Restriction endonucleases | An enzyme that cleaves a DNA duplex molecule at a particular base sequence, usually within or near a palindromic sequence |
| iii. | Synthesizing enzymes | Reverse transcriptase; DNA polymerase |
Reverse transcriptase: A viral enzyme that is capable of converting their RNA genome into a DNA copy DNA polymerase: It synthesizes DNA strand only in the 5′- to-3′ direction |
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संबंधित प्रश्न
Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
What is plasmid?
From the following list of cloning vectors used in rDNA technology identify the vectors that are the most commonly used.
Plasmids, bacteriophages (Ml3, lambda virus), cosmid, phagemid, BAC (bacterial artificial chromosome), YAC (yeast artificial chromosome), transposons, baculoviruses, and mammalian artificial chromosomes (MACs)
Match the Column I (Restriction enzyme) with Column II (Recognition sequence) and select the correct option.
| column I (Restriction Enzyme) | column II (Recognition sequence) | ||
| i. | Alu | P. | 5'---G- ↓-G-A-T-C-C---3' 3'---C-C-T-A-G- -G---5 |
| ii. | BamHI | Q. | 5'--- A-G- ↓ -C-T---3' 3' ---T-C- G-A--- 5' |
| iii. | Eco RI | R. | 5'--G-T-C- ↓ -G-A-C--3' I 3'--C-A-G-t-C-T-G--5' |
| iv. | HindII | S. | 5'---G- ↓ -A-A-T-T-C---3' 3'---C-T-T-A-A- -G---5' |
____________ cuts the DNA within the specific positions.
Which of the following is the right combination of primers in a PCR to amplify the DNA product?
Identify the CORRECT order of steps in Polymerase Chain Reaction (PCR).
Annealing step of PCR operates at ______ °C.
Write steps involved in gene amplification.
Name the enzymes that are called molecular scissors.
