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प्रश्न
Polymerase chain reaction is most useful in ______.
विकल्प
DNA amplification
DNA synthesis
protein synthesis
selective replication of DNA
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उत्तर
Polymerase chain reaction is most useful in DNA amplification.
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संबंधित प्रश्न
Bt-cotton shows adverse effect on the population of which butterfly?
From the following which one is NOT a feature of the plasmids?
The time taken to complete one cycle of PCR is around ______.
The following identifies the major features of technology that differentiate modem biotechnology from classical biotechnology.
i. Capability of science to change the genetic material for getting new specific products through rDNA technology, polymerase chain reaction (PCR), microarrays, cell culture and fusion, and bioprocessing.
ii. Ownership of technology and its sociopolitical impact.
iii. Modem biotechnology makes use of only fermentation technology.
iv. Classical biotechnology relies only on principles of plant and animal tissue culture.
The source of taq Polymerase used in PCR is a ______.
| Oil spill is a major environmental issue. It has been found that different strains of Pseudomonas bacteria have genes to break down the four major groups of hydrocarbons in oil. Trials are underway to use different biotechnological tools to incorporate these genes and create a genetically engineered strain of Pseudomonas - a ‘super-bug’, to break down the four major groups of hydrocarbons in oil. Such bacteria might be sprayed onto surfaces polluted with oil to clean thin films of oil. |
(a) List two advantages of using bacteria for such biotechnological studies?
(b) For amplification of the gene of interest PCR was carried out. The PCR was run with the help of polymerase which was functional only at a very low temperature. How will this impact the efficiency of the PCR? Justify.
(c) If such bacteria are sprayed on water bodies with oil spills, how will this have a positive or negative effect on the environment? Discuss.
The plasmid of Agrobacterium tumefaciens that is now modified as a cloning vector is ______.
To be a cloning vector, a plasmid require ______.
Write steps involved in gene amplification.
______technique is used for in vitro gene cloning or gene multiplication.
