Advertisements
Advertisements
प्रश्न
Explain types of enzymes used in biotechnology?
Advertisements
उत्तर
| No | Type of enzyme | Examples | Functions |
| i. | Lysing enzyme | Lysozyme | Digesting bacterial cell walls |
| ii. | Cleaving enzymes (Molecular scissors) | Restriction endonucleases | An enzyme that cleaves a DNA duplex molecule at a particular base sequence, usually within or near a palindromic sequence |
| iii. | Synthesizing enzymes | Reverse transcriptase; DNA polymerase |
Reverse transcriptase: A viral enzyme that is capable of converting their RNA genome into a DNA copy DNA polymerase: It synthesizes DNA strand only in the 5′- to-3′ direction |
APPEARS IN
संबंधित प्रश्न
The enzyme nuclease hydrolyses ______ of polynucleotide chain of DNA.
Match the column I (PCR steps) and Column II (Temperature). Select the correct option.
| Column I (PCR steps) | Column II (Temperature) | ||
| i. | Polymerization | P. | 40 - 60°C |
| ii. | Denaturation | Q. | 70 - 75°C |
| iii. | Primer annealing | R. | 90 - 98°C |
| S. | 28 - 37°C |
Lytic cycle involves bacteriophage and ____________.
Which of the following viruses has the capacity to produce lysozymes?
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
Which vector is mostly used in rDNA technology in plants?
Which of the following enzyme catalyses the removal of nucleotides from the end of DNA molecules?
To be a cloning vector, a plasmid require ______.
In vitro amplification of DNA segments is called______.
When the phage DNA enters the host bacterium, the host cell protects itself from the viral DNA attacks with the help of ______ enzyme.
