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प्रश्न
Explain types of enzymes used in biotechnology?
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उत्तर
| No | Type of enzyme | Examples | Functions |
| i. | Lysing enzyme | Lysozyme | Digesting bacterial cell walls |
| ii. | Cleaving enzymes (Molecular scissors) | Restriction endonucleases | An enzyme that cleaves a DNA duplex molecule at a particular base sequence, usually within or near a palindromic sequence |
| iii. | Synthesizing enzymes | Reverse transcriptase; DNA polymerase |
Reverse transcriptase: A viral enzyme that is capable of converting their RNA genome into a DNA copy DNA polymerase: It synthesizes DNA strand only in the 5′- to-3′ direction |
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संबंधित प्रश्न
Explain what are the desirable characteristics of an ideal cloning vector used in rDNA technology.
The bacterium which causes a plant disease called crown gall is _______________.
A PCR machine can raise the temperature up to 100°C but after that, it is not able to lower the temperature below 70°C automatically. Which step of PCR will be hampered first in this faulty machine? Explain why?
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
Following are the four statements regarding separation of DNA fragments using Gel electrophoresis. Identify the INCORRECT statements.
i. DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal.
ii. DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
iii. Smaller the size of DNA fragment, larger is the distance it travels through it.
iv. Pure DNA can be visualized directly by exposing to UV radiation.
Match the column I (PCR steps) and Column II (Temperature). Select the correct option.
| Column I (PCR steps) | Column II (Temperature) | ||
| i. | Polymerization | P. | 40 - 60°C |
| ii. | Denaturation | Q. | 70 - 75°C |
| iii. | Primer annealing | R. | 90 - 98°C |
| S. | 28 - 37°C |
Exonuclease performs the function of ____________.
Which molecules are called molecular scissors? Why are they called so?
Importance of PCR.
The restriction enzymes used as molecular scissors are type of ______.
