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प्रश्न
Explain types of enzymes used in biotechnology?
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उत्तर
| No | Type of enzyme | Examples | Functions |
| i. | Lysing enzyme | Lysozyme | Digesting bacterial cell walls |
| ii. | Cleaving enzymes (Molecular scissors) | Restriction endonucleases | An enzyme that cleaves a DNA duplex molecule at a particular base sequence, usually within or near a palindromic sequence |
| iii. | Synthesizing enzymes | Reverse transcriptase; DNA polymerase |
Reverse transcriptase: A viral enzyme that is capable of converting their RNA genome into a DNA copy DNA polymerase: It synthesizes DNA strand only in the 5′- to-3′ direction |
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संबंधित प्रश्न
The biological scissor is ______.
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
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Match the List I with List II and List ill. Select the correct option.
| List I | List II | List ill | |||
| i. | Hind III | a. | Agarose ge | 1. | Six base pairs |
| ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marke |
| iii. | T-DNA | c. | Ampicillin | 3. | Elution |
| iv. | DNA | d. | Recognition sequenc | 4. | Transgenic plant |
Match the column I (PCR steps) and Column II (Temperature). Select the correct option.
| Column I (PCR steps) | Column II (Temperature) | ||
| i. | Polymerization | P. | 40 - 60°C |
| ii. | Denaturation | Q. | 70 - 75°C |
| iii. | Primer annealing | R. | 90 - 98°C |
| S. | 28 - 37°C |
A hind of biotechnology involving manipulation of DNA is ______.
The PCR technique is used for ______.
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The manipulation of genetic material towards a desired end using "in-vitro" process is called______.
